The effects of Porphyromonas gingivalis on the cell cycle progression of human gingival epithelial cells.
The effects of Porphyromonas gingivalis on the cell cycle progression of human gingival epithelial cells.
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DOI：
10.1111/odi.12081
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发表时间：
2014
期刊：
影响因子：
3.8
通讯作者：
C. Pan;Xue-bin Xu;L. Tan;Li Lin;Yaping Pan
中科院分区：
文献类型：
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作者：
C. Pan;Xue-bin Xu;L. Tan;Li Lin;Yaping Pan
目的牙龈卟啉单胞菌是牙周病发生和发展的主要病原体。细菌和牙龈上皮细胞之间的相互作用或串扰驱使细菌操纵细胞周期，以有利于细菌在宿主内的存活和毒力表达。本研究旨在剖析牙龈卟啉单胞菌对人类牙龈上皮细胞细胞周期的影响。材料和方法我们建立了牙龈卟啉单胞菌侵入IHGE细胞的模型。采用流式细胞仪分析人牙龈上皮细胞的细胞周期分布。分别通过实时PCR和Western blot检测Cyclin D和Cyclin E mRNA和蛋白。结果牙龈卟啉单胞菌诱导的细胞生长促进与细胞周期G1期的加速相关。感染后 6 至 12 小时，Cyclin D1 mRNA 水平显着上调。细胞周期蛋白 E 蛋白和 mRNA 水平在入侵后 10 和 12 小时升高。结论 我们证实牙龈卟啉单胞菌通过促进 G1/S 转变，涉及细胞周期蛋白 D 和细胞周期蛋白 E 的上调，显着增强 IHGE 细胞增殖。
OBJECTIVE Porphyromonas gingivalis is a major pathogen in the development and progression of periodontal disease. The interactions or cross-talk between bacteria and gingival epithelial cells drive bacteria to manipulate the cell cycle to favor bacterial survival and virulence expression within the host. This study aims to dissect the effects of P. gingivalis on the cell cycle in human gingival epithelial cells. MATERIALS AND METHODS We established a model of P. gingivalis invading IHGE cells. The cell cycle distribution of human gingival epithelial cells was analyzed by flow cytometry. Cyclin D and cyclin E mRNA and protein were detected by real-time PCR and Western blot, respectively. RESULTS Porphyromonas gingivalis-induced facilitation of cell growth was correlated with the acceleration of G1 phase of cell cycle. Cyclin D1 mRNA levels were significantly upregulated from 6 to 12 h after infection. Cyclin E protein and mRNA levels were elevated at 10 and 12 h after invasion. CONCLUSIONS We confirmed that P. gingivalis significantly enhances IHGE cell proliferation by promoting the G1/S transition, involving the up-regulation of cyclin D and cyclin E.