Recombinant histamine-releasing factor enhances IgE-dependent IL-4 and IL-13 secretion by human basophils.
Recombinant histamine-releasing factor enhances IgE-dependent IL-4 and IL-13 secretion by human basophils.
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DOI：
10.4049/jimmunol.159.1.447
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发表时间：
1997-07
影响因子：
4.4
通讯作者：
J. Schroeder;L. M. Lichtenstein;S. Macdonald
中科院分区：
文献类型：
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作者：
J. Schroeder;L. M. Lichtenstein;S. Macdonald
已知人重组组胺释放因子 (HrHRF) 在需要表达特定类型 IgE（称为 IgE+）的反应中直接刺激选定特应性供体的嗜碱性粒细胞释放组胺和 IL-4。在这项研究中，HrHRF 被证明会影响 IgE 介导的 IL-4、IL-13 和组胺从正常情况下不释放该蛋白的嗜碱性粒细胞（即表达 IgE- 的细胞）的释放。用几种不同浓度的 HrHRF 引发 15 分钟，在用抗 IgE Ab (10 ng/ml) 激活后 4 小时后，以剂量依赖性方式增强嗜碱性粒细胞分泌 IL-4 和组胺。 HrHRF 引发的这种效应也发生在用 1 或 100 ng/ml 抗 IgE Ab 激活的培养物中。在用抗 IgE Ab 刺激 16 至 20 小时的培养物中，通过 HrHRF 引发类似地增强了 IL-13 蛋白的分泌。然而，用 HrHRF 引发并用不依赖 IgE 的促分泌素 FMLP 激活的嗜碱性粒细胞的组胺或细胞因子的分泌没有明显变化。这些发现表明，HrHRF 通过与特定受体结合来改变嗜碱性粒细胞对 IgE 依赖性分泌的反应，从而扩大了该蛋白在慢性过敏性炎症中的可能作用。
Human recombinant histamine-releasing factor (HrHRF) is known to directly stimulate histamine release and IL-4 secretion from basophils of selected atopic donors in a reaction requiring the expression of a particular type of IgE, referred to as IgE+. In this study, HrHRF is shown to affect the IgE-mediated release of IL-4, IL-13, and histamine from basophils not normally releasing to this protein (i.e, those expressing IgE-). Priming with several different concentrations of HrHRF for 15 min enhanced basophil secretion of IL-4 and histamine after 4 h in a dose-dependent fashion following activation with anti-IgE Ab (10 ng/ml). This effect of HrHRF priming also occurred in cultures activated with 1 or 100 ng/ml of anti-IgE Ab. The secretion of IL-13 protein was enhanced similarly by HrHRF priming in cultures stimulated for 16 to 20 h with anti-IgE Ab. There were, however, no apparent changes in the secretion of histamine or cytokine by basophils primed with HrHRF and activated with the IgE-independent secretogogue, FMLP. These findings suggest that HrHRF modifies the response of basophils for IgE-dependent secretion by binding to a specific receptor, broadening the possible role of this protein in chronic allergic inflammation.