SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LIPID HYDROPEROXIDES
SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF LIPID HYDROPEROXIDES
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DOI：
10.1016/s0003-2697(79)80003-2
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发表时间：
1979-01-01
影响因子：
2.9
通讯作者：
GEBICKI, JM
中科院分区：
文献类型：
--
作者：
HICKS, M;GEBICKI, JM
A modification of the iodine technique for peroxide determination is described, in which the reaction is carried out in deaerated methanol:acetic acid solution in a spectrophotometer cuvette. Measurements of the triiodide ions released are carried out either at 290 or 360 nm. In this solvent, the molar extinction coefficients are .epsilon.290 = 4.41 .times. 104 and .epsilon.360 = 2.80 .times. 104 l mol-1 cm-1. Calibration with standard peroxide solutions showed that 1 mol of I3- is produced for every peroxide group added. In its present form, the method can be used to measure 1 nEq of lipid hydroperoxide.
