Single-cell transcriptomics: a novel precision medicine technique in nephrology.
Single-cell transcriptomics: a novel precision medicine technique in nephrology.
复制标题
单细胞转录组学：肾脏病学中的一种新型精准医学技术。
DOI：
10.3904/kjim.2020.415
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发表时间：
2021-05
期刊：
影响因子：
--
通讯作者：
Park J
中科院分区：
文献类型：
--
作者：
Kim J;Park J
由于肾脏的复杂结构和功能，肾脏疾病的发生机制尚不清楚。特别是，总体水平上的转录组学方法无法区分导致疾病发展的原发性自主反应和继发性细胞非自主反应。单细胞分析技术可以克服测量异质性细胞群固有的局限性，并阐明肾脏生物学和疾病发病机制的核心问题。单细胞测序有助于识别疾病相关的生物标志物和途径，对患者进行分层，并决定适当的治疗方法。在这里，我们回顾了各种单细胞分析技术和单细胞转录组学研究在肾病学领域进行。此外，我们还讨论了基于单细胞分析的肾内科精准医学的未来前景。
Due to the complex structure and function of the kidneys, the mechanism of kidney disease is unclear. In particular, transcriptomics approaches at the bulk level are unable to differentiate primary autonomous responses, which lead to disease development, from secondary cell non-autonomous responses. Single-cell analysis techniques can overcome the limitations inherent in the measurement of heterogeneous cell populations and clarify the central issues in kidney biology and disease pathogenesis. Single-cell sequencing helps in identifying disease-related biomarkers and pathways, stratifying patients, and deciding on appropriate treatment methods. Here we review a variety of single-cell analysis techniques and single-cell transcriptomics studies performed in the field of nephrology. Moreover, we discuss the future prospects of single-cell analysis-based precision medicine in nephrology.