Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse genetics
Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse genetics
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DOI：
10.1016/j.virol.2005.07.011
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发表时间：
2005-10-10
期刊：
影响因子：
3.7
通讯作者：
Chen, HL
中科院分区：
文献类型：
--
作者：
Tian, GB;Zhang, SH;Chen, HL
我们通过基于质粒的反向遗传学产生了高生长的 H5N1/PR8 病毒。 HA基因的多个碱性氨基酸相关的毒力被去除，所得病毒对鸡和鸡蛋来说是减毒的。从此病毒制备了福尔马林灭活的油乳剂疫苗。当 SPF 鸡接种 0.3 ml 疫苗时，接种后 1 周 (p.v.) 可检测到血凝素抑制 (HI) 抗体，并在 p.v. 6 周时达到峰值 101log2。然后在 43 周 p.v. 时缓慢下降至 4log2。挑战研究在 p.v. 2、3 和 43 周进行。表明所有鸡都完全免受疾病症状和死亡的影响。鸭和鹅在 p.v. 3 周后完全免受高致病性 H5N1 病毒攻击。通过检测现场接种禽类的HI抗体，考察了鸭、鹅的保护性免疫力持续时间，结果表明，鹅接种3剂疫苗可产生34周的保护期，鸭接种2剂剂可产生超过52周的保护期。我们首先报道了一种源自高生长 H5N1 疫苗的油乳灭活疫苗在鸡中诱导了大约 10 个月的保护性免疫，并证明油乳灭活禽流感疫苗对鹅和鸭具有免疫原性。这些结果为应用疫苗控制家禽（包括鸡和家养水禽）H5N1禽流感提供了有用的信息。 (c) 2005 Elsevier Inc. 保留所有权利。
We generated a high-growth H5N1/PR8 virus by plasmid-based reverse genetics. The virulence associated multiple basic amino acids of the HA gene were removed, and the resulting virus is attenuated for chickens and chicken eggs. A formalin-inactivated oil-emulsion vaccine was prepared from this virus. When SPF chickens were inoculated with 0.3 ml of the vaccine, the hemagglutinin-inhibition (HI) antibody became detectable at 1 week post-vaccination (p.v.) and reached a peak of 101log2 at 6 weeks p.v. then slowly declined to 4log2 at 43 weeks p.v. Challenge studies performed at 2, 3 and 43 weeks p.v. indicated that all of the chickens were completely protected from disease signs and death. Ducks and geese were completely protected from highly pathogenic H5N1 virus challenge 3 weeks p.v. The duration of protective inummity in ducks and geese vas investigated by detecting the HI antibody of the field vaccinated birds, and the results indicated that 3 doses of the vaccine inoculation in geese could induce a 34 weeks protection, while 2 doses induced more than 52 weeks protection in ducks. We first reported that an oil-emulsion inactivated vaccine derived from a high-growth H5N1 vaccine induced approximately 10 months of protective immunity in chickens and demonstrated that the oil-emulsion inactivated avian influenza vaccine is immunogenic for geese and ducks. These results provide useful information for the application of vaccines to the control of H5N1 avian influenza in poultry, including chickens and domestic waterfowl. (c) 2005 Elsevier Inc. All rights reserved.