Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone inHyphomicrobium denitrificansFJNU-6
Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone inHyphomicrobium denitrificansFJNU-6
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提高脱氮丝微菌 FJNU-6 中吡咯喹啉醌补料分批产量的两阶段供氧策略
DOI：
10.1007/s00253-020-10690-0
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发表时间：
2020-06-12
影响因子：
5
通讯作者：
Ke, Chongrong
中科院分区：
文献类型：
--
作者：
Liu, Mengsu;Yang, Xinwei;Ke, Chongrong
氧是吡咯喹啉醌（PQQ）生物合成的重要参数。本研究首次系统地研究了供氧条件对菌丝微菌FJNU-6生物合成PQQ的影响。通过对5L发酵系统中不同供氧水平（0 ~ 60%）下的比细胞生长速率（μx）和比PQQ生成速率（μp）的动力学分析，提出了一种新的两阶段供氧策略，以提高PQQ的产量和产率。此外，在整个过程中，参与甲醇氧化和PQQ生物合成的基因的转录进行了分析，概述了氧气供应对细胞代谢的影响。在5L发酵罐中，当初始甲醇消耗完后，以0- 1g/L的甲醇浓度持续补料，发酵140 h后，PQQ的浓度和产量分别达到1070 mg/L和7.64mg/L/h。本研究提出的两段供氧策略为PQQ的工业化生产提供了一种有效、经济的供氧策略。关键点·开发了一种新的两阶段供氧策略，以提高PQQ的产量和生产率。·参与甲醇氧化和PQQ生物合成的基因的转录受到氧供应变化的调节。该研究为PQQ的工业化或大规模生产提供了一种有效且经济的策略。
Oxygen is a vital parameter for pyrroloquinoline quinone (PQQ) biosynthesis. In this study, the effects of oxygen supply on the biosynthesis of PQQ were first investigated systematically with Hyphomicrobium denitrificans FJNU-6. Following a kinetic analysis of the specific cell growth rate (μx) and specific PQQ formation rate (μp) in 5 L benchtop fermentation systems at various oxygen supply levels ranging from 0 to 60%, a novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity. Moreover, the transcription of genes involved in methanol oxidation and PQQ biosynthesis was analyzed throughout the process to outline the effect of oxygen supply on cell metabolism. Furthermore, with constant feeding of methanol at 0–1 g/L after the initial methanol was consumed completely, the PQQ concentration and productivity reached 1070 mg/L and 7.64 mg/L/h, respectively, after 140 h in a 5-L fermenter. The two-stage oxygen supply strategy developed in this study provides an effective and economical strategy for the industrial production of PQQ. Key Points • A novel, two-stage oxygen supply strategy was developed for enhancing PQQ production and productivity. •The transcription of genes involved in methanol oxidation and PQQ biosynthesis was regulated by changes in oxygen supply. • This study offers an effective and economical strategy for industrial or large-scale production of PQQ.