SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice
SIRT5 deacetylates and activates urate oxidase in liver mitochondria of mice
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DOI：
10.1016/j.febslet.2012.10.009
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发表时间：
2012-11-30
期刊：
影响因子：
3.5
通讯作者：
Inagaki, Nobuya
中科院分区：
文献类型：
--
作者：
Nakamura, Yasuhiko;Ogura, Masahito;Inagaki, Nobuya
通过双向电泳法比较SIRT5过表达转基因小鼠(SIRT5TG)和野生型小鼠肝脏中的线粒体蛋白，确定尿酸氧化酶(UOX)是SIRT5的靶标。SIRT5转基因小鼠肝脏中UOX乙酰化水平约为野生型小鼠的一半，UOX活性显著增加。在体外合成的UOX蛋白在与野生型小鼠肝脏线粒体孵育时被乙酰化，但在与SIRT5转基因小鼠肝脏孵育时水平较低。这些结果表明，SIRT5通过去乙酰化激活小鼠肝线粒体中的UOX。
We identified urate oxidase (UOX) as a target of SIRT5 by comparing mitochondrial proteins in livers of SIRT5-overexpressing transgenic (SIRT5 Tg) and wild-type mice by using two-dimensional electrophoresis. Acetylation levels of UOX in liver of SIRT5 Tg mice were approximately half of those in wild-type mice, and UOX activity was significantly increased. In vitro-synthesized UOX protein was acetylated when incubated with mitochondria from wild-type mice liver but the levels were less when incubated with those from SIRT5 Tg mice liver. These results suggest that SIRT5 activates UOX through deacetylation in mouse liver mitochondria.