Molecular characterization of TLR22 and its role in immunological modification of the brood pouch of the lined seahorse, Hippocampus erectus
Molecular characterization of TLR22 and its role in immunological modification of the brood pouch of the lined seahorse, Hippocampus erectus
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DOI：
10.1016/j.aquaculture.2021.736628
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发表时间：
2021-06
期刊：
影响因子：
4.5
通讯作者：
Yongli Wu;Huixian Zhang;Bo Zhang;Q. Lin;Yali Liu
中科院分区：
文献类型：
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作者：
Yongli Wu;Huixian Zhang;Bo Zhang;Q. Lin;Yali Liu
海龙科是一个特殊的鱼类家庭，其中怀孕的男性孵化发育中的胚胎育袋，功能相当于哺乳动物的子宫。妊娠期间育儿袋中的免疫修饰对于成功妊娠至关重要。以往的研究表明，硬骨鱼特异性Toll样受体22（TLR22）可以识别病毒和细菌。为了评估TLR22在同种异体移植耐受性和抗细菌（和抗病毒）保护中的作用，在已经进化出复杂和完全封闭的育袋的有衬里的海马（Hippocampus erectus）中表征了TLR22基因。在本研究中，tlr22在育袋中有特异性表达。有趣的是，在妊娠过程中，tlr22在育儿袋中的表达逐渐减少。此外，TLR下游信号分子的表达受妊娠阶段的影响，提示TLR 22及其下游信号分子可能参与了妊娠期卵囊的免疫耐受和胚胎排斥。进一步研究TLR22在注射LPS和Poly（I：C）后的育袋中的免疫保护作用表明，攻击后育袋中TLR22的表达上调。此外，下游TLR信号分子的表达也发生了改变，表明tattlr22和TLR信号分子参与了育袋中的抗病毒和抗菌免疫应答。总之，我们的研究结果提高了对育袋中免疫修饰的理解，并可能为海龙类雄性妊娠期间的免疫调节提供新的见解。
Syngnathidae is a peculiar fish family in which pregnant males incubate developing embryos in a brood pouch which is functionally equivalent to the mammalian uterus. Immunological modifications in the brood pouch during gestation are vital for a successful pregnancy. Previous studies revealed the teleost-specific Toll-like receptor 22 (TLR22) could recognize viruses and bacteria. To evaluate the role of TLR22 in allograft tolerance and antibacterial (and antiviral) protection in the brood pouch, thetlr22gene was characterized in the lined seahorse (Hippocampus erectus), which has evolved complex and fully enclosed brood pouches. In this study,tlr22showed specific expression in the brood pouch. Interestingly, expression oftlr22in the pouch decreased progressively during the course of pregnancy. In addition, the expression of downstream TLR signaling molecules was influenced by pregnancy stages, suggesting that TLR22 and downstream TLR signaling molecules might be involved in immunological tolerance and embryo rejection in the brood pouch during pregnancy. Further investigation of role of TLR22 in immunological protection in brood pouches following the injection of LPS and Poly(I:C) showed thattlr22expression in the pouch was upregulated after the challenge. In addition, the expression of downstream TLR signaling molecules was altered, indicating thattlr22and TLR signaling molecules are involved in antiviral and antibacterial immune responses in the brood pouch. Taken together, our results improve the understanding of immunological modifications in the brood pouch and may provide new insights into immune regulation during male pregnancy in syngnathids.