Gelatin methacrylate (GelMA) mediated electrochemical DNA biosensor for DNA hybridization.
Gelatin methacrylate (GelMA) mediated electrochemical DNA biosensor for DNA hybridization.
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DOI：
10.1016/j.bios.2014.09.060
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发表时间：
2015-02
影响因子：
12.6
通讯作者：
S. N. Topkaya
中科院分区：
文献类型：
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作者：
S. N. Topkaya
在这项研究中，开发了一种使用甲基丙烯酸明胶（GelMA）修饰电极检测DNA杂交的电化学生物传感器系统。使用循环伏安法 (CV) 和电化学阻抗谱 (EIS) 研究了作为功能平台的 GelMA 修饰铅笔石墨电极 (PGE) 的电化学行为，并与裸 PGE 的电化学行为进行了比较。在溶液相中实现杂交并评估鸟嘌呤氧化信号的变化。 +1.0 V 左右鸟嘌呤氧化峰值电流的降低被用作 DNA 杂交的指标。此外，更有趣的是，通过将不同的寡核苷酸（例如探针、杂交体和控制序列）固定到电极表面，GelMA 在 +0.7 V 左右的固有氧化峰发生了显着变化。这是首次使用 GelMA 作为电化学生物传感器系统的一部分的研究。就使用 GelMA 作为新的 DNA 杂交指示剂而言，结果非常有希望。此外，GelMA 修饰电极通过为生物识别层提供机械支撑，可用于检测超少量寡核苷酸。目前该方法的检测限为10-12mol。与裸电极相比，使用 GelMA 杂交的信号抑制从 50% 增加到 93%，有利于杂交检测。
In this study, an electrochemical biosensor system for the detection of DNA hybridization by using gelatin methacrylate (GelMA) modified electrodes was developed. Electrochemical behavior of GelMA modified Pencil Graphite Electrode (PGE) that serve as a functional platform was investigated by using Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) and compared with those of the bare PGE. Hybridization was achieved in solution phase and guanine oxidation signal changes were evaluated. The decrease in the guanine oxidation peak currents at around +1.0 V was used as an indicator for the DNA hybridization. Also, more interestingly GelMA intrinsic oxidation peaks at around +0.7 V changed substantially by immobilization of different oligonucleotides such as probe, hybrid and control sequences to the electrode surface. It is the first study of using GelMA as a part of an electrochemical biosensor system. The results are very promising in terms of using GelMA as a new DNA hybridization indicator. Additionally, GelMA modified electrodes could be useful for detecting ultra low quantity of oligonucleotides by providing mechanical support to the bio-recognition layer. The detection limit of this method is at present 10-12mol. Signal suppressions were increased from 50% to 93% for hybrid with using GelMA when it was compared to bare electrode which facilitates the hybridization detection.