Recent emergence of new variants of Yersinia pestis in Madagascar
Recent emergence of new variants of Yersinia pestis in Madagascar
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DOI：
10.1128/jcm.35.11.2826-2833.1997
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发表时间：
1997-11-01
影响因子：
9.4
通讯作者：
Carniel, E
中科院分区：
文献类型：
--
作者：
Guiyoule, A;Rasoamanana, B;Carniel, E
鼠疫耶尔森氏菌是鼠疫的病原体，至少造成了三次大流行。在1894年在香港开始的最后一次大流行期间，该微生物定殖到新的、以前未受损害的地理区域，在那里它已经很好地建立。鼠疫菌株引入到一个新的环境下刚刚在世纪前，并遵循流行病学的任何新的遗传变异检测。研究了1939年至1996年从马达加斯加不同地区分离出来的鼠疫菌，这些鼠疫菌主要是造成人类淋巴腺鼠疫和肺鼠疫的病例。我们的主要基因分型方法是rRNA基因分型（核糖体分型），这是一种有效的Y染色体分型方法。不同地理来源的鼠疫菌株，我们报告，所有研究的Y。1982年以前在马达加斯加分离的鼠疫菌株是经典的核糖体型B，该核糖体型归属于Y. 1982年、1983年和1994年，分别在该岛的高原地区分离出具有新核糖体型的菌株，命名为R、Q和T，对其他基因型特征如NotI基因组限制性图谱和EcoRV质粒限制性图谱的分析表明，新的变异体也可以通过以下方式区分：对这些新的变异体的跟踪表明，核糖核酸型Q和R的菌株已经在其生态系统中建立良好，并且具有传播到新的地理区域并取代原始经典菌株的趋势。
Yersinia pestis, the causative agent of plague, has been responsible far at least three pandemics. During the last pandemic, which started in Hong Kong in 1894, the microorganism colonized new, previously unscathed geographical areas where it has become well established, The aim of this longitudinal study was to investigate the genetic stability of Y. pestis strains introduced into a new environment just under a century ago and to follow the epidemiology of any new genetic variant detected, In the present study, 187 strains of Y. pestis isolated between 1939 and 1996 from different regions of Madagascar and responsible mainly far human cases of bubonic and pneumonic plague were studied. Our principal genotyping method nas rRNA gene profiling (ribotyping), which has previously been shown to be an effective scheme for typing Y. pestis strains of different geographical origins, We report that all studied Y. pestis strains isolated in Madagascar before 1982 were of classical ribotype B, the ribotype attributed to the Y. pestis clone that spread around the world during the third pandemic, In 1982, 1983, and 1994, strains with new ribotypes, designated R, Q, and T, respectively, were isolated on the high-plateau region of the island, Analysis of other genotypic traits such as the NotI genomic restriction profiles and the EcoRV plasmid restriction profiles revealed that the new variants could also be distinguished by specific genomic and/or plasmid profiles, A follow-up of these new variants indicated that strains of ribotypes Q and R have become well established in their ecosystem and ha je a tendency to spread to new geographical areas and supplant the original classical strain.