High Omega-3 Polyunsaturated Fatty Acids in fat-1 Mice Reduce Inflammatory Pain.
High Omega-3 Polyunsaturated Fatty Acids in fat-1 Mice Reduce Inflammatory Pain.
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fat-1 小鼠中的高 Omega-3 多不饱和脂肪酸可减轻炎症疼痛。
DOI：
10.1089/jmf.2016.3871
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发表时间：
2017
影响因子：
2.4
通讯作者：
Sun Yeul Lee
中科院分区：
文献类型：
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作者：
Enji Zhang;Jwa;Nara Shin;Yuhua Yin;Yongshan Nan;Y. Xu;Jinpyo Hong;T. Hsu;Woosuk Chung;Youngkwon Ko;Won;Kyu Lim;D. Kim;Sun Yeul Lee
ω-3和ω-6多不饱和脂肪酸（PUFA），如α-亚麻酸和亚油酸，是哺乳动物的必需脂肪酸，因为它们不能从头合成。然而，fat-1转基因小鼠可以从omega-6 PUFA合成omega-3 PUFA，而无需膳食补充omega-3，导致各种组织中大量的omega-3 PUFA积累。在这项研究中，我们使用fat-1转基因小鼠来研究omega-3 PUFAs在炎症疼痛反应中的作用。高ω-3 PUFA组织含量减弱福尔马林诱导的疼痛敏感性、小胶质细胞活化、诱导型一氧化氮合酶表达和N-甲基-d-天冬氨酸（NMDA）受体亚基NR 2B的磷酸化。我们的研究结果表明，ω-3多不饱和脂肪酸水平升高抑制脊髓背角的NMDA受体活性，并通过调节脊髓背角的信号传递来调节炎性疼痛的传递，导致化学诱导的炎性疼痛的衰减。
Omega-3 and omega-6 polyunsaturated fatty acids (PUFAs), such as α-linolenic and linoleic acids, are essential fatty acids in mammals, because they cannot be synthesized de novo. However, fat-1 transgenic mice can synthesize omega-3 PUFAs from omega-6 PUFAs without dietary supplementation of omega-3, leading to abundant omega-3 PUFA accumulation in various tissues. In this study, we used fat-1 transgenic mice to investigate the role of omega-3 PUFAs in response to inflammatory pain. A high omega-3 PUFA tissue content attenuated formalin-induced pain sensitivity, microglial activation, inducible nitric oxide synthase expression, and the phosphorylation of NR2B, a subunit of the N-methyl-d-aspartate (NMDA) receptor. Our findings suggest that elevated omega-3 PUFA levels inhibit NMDA receptor activity in the spinal dorsal horn and modulate inflammatory pain transmission by regulating signal transmission at the spinal dorsal horn, leading to the attenuation of chemically induced inflammatory pain.