Isolation and functional analysis of human HMBOX1, a homeobox containing protein with transcriptional repressor activity
Isolation and functional analysis of human HMBOX1, a homeobox containing protein with transcriptional repressor activity
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DOI：
10.1159/000093328
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Yu, L.
中科院分区：
文献类型：
--
作者：
Chen, S.;Saiyin, H.;Yu, L.
我们从胰腺 cDNA 文库中鉴定并分离出一种新的人类基因 HMBOXI（包含 1 的同源框）。人HMBOXI在18种组织中广泛表达，其中在胰腺中高表达。根据基因组数据库，HMBOXI位于8p12.3和8p21.1的边界处。 HMBOXI 蛋白在人类、小鼠、大鼠、鸡和非洲爪蟾中高度保守。系统发育树显示 HMBOXI 可能代表 HNF（肝细胞核因子）转录因子中的一个独特群体。功能性 HMBOXI::EGFP（增强型绿色荧光蛋白）融合蛋白显示 HMBOX1 在细胞质中的积累量多于在细胞核中的积累量。用pM-HMBOX1质粒和报告质粒pGAL4(5)tkLUC共转染HEK-293T细胞表明HMBOX1是转录抑制子。小鼠组织石蜡切片的原位杂交表明，Hmbox1在胰腺中广泛表达，并且在大脑皮层、海马和下丘脑中也能检测到该基因的表达。版权所有 (c) 2006 S. Karger AG，巴塞尔。
We have identified and isolated a novel human gene, HMBOXI (homeobox containing 1) from a pancreatic cDNA library. Human HMBOXI is widely expressed in 18 tissues, and it is highly expressed in pancreas. According to the genome database, HMBOXI is located at the boundary of 8p12.3 and 8p21.1. HMBOXI proteins are highly conserved in human, mouse, rat, chicken and Xenopus laevis. A phylogenetic tree shows that HMBOXI may represent a distinct group in HNF (Hepatocyte Nuclear Factor) transcriptional factors. Functional HMBOXI::EGFP (enhanced green fluorescent protein) fusion protein revealed that HMBOX1 accumulated more in cytoplasm than in nucleus. Co-transfection of HEK-293T cells with pM-HMBOX1 plasmid and reporter plasmid pGAL4(5)tkLUC indicates that HMBOX1 is a transcription repressor. In situ hybridization on paraffin sections of mouse tissues demonstrated that Hmbox1 is widely expressed in pancreas and the expression of this gene can also be detected in pallium, hippocampus and hypothalamus. Copyright (c) 2006 S. Karger AG, Basel.