High-content screening as a universal tool for fingerprinting of cytotoxicity of nanoparticles
High-content screening as a universal tool for fingerprinting of cytotoxicity of nanoparticles
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DOI：
10.1021/nn7004393
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发表时间：
2008-05-01
期刊：
影响因子：
17.1
通讯作者：
Kotov, Nicholas A.
中科院分区：
文献类型：
--
作者：
Jan, Edward;Byrne, Stephen J.;Kotov, Nicholas A.
纳米生物技术的最新进展和进展已经证明许多纳米颗粒（NPs）作为潜在的和新型的药物递送载体、治疗剂和造影剂以及用于生物成像的发光生物标记物。基于纳米粒子的新生物医学应用的出现意味着需要理解，比较和管理其细胞毒性。在这项研究中，我们证明了使用高含量筛选试验（HCA）作为一种通用的工具，探测纳米粒子的细胞毒性，特别是碲化镉量子点（CdTe量子点）和金纳米粒子（Au纳米粒子）在NG 108 -15小鼠神经母细胞瘤细胞和HepG 2人肝癌细胞。神经细胞对NP诱导的细胞毒性特别感兴趣，因为神经成像和接口所需的材料的光学和电学功能与许多NP的性质很好地匹配。此外，神经元的细胞形态特别适合于自动化高含量筛选。HepG 2细胞代表了高含量筛选研究的良好模型，因为它们通常在药物研究中用作人肝细胞的替代物。我们发现，碲化镉量子点诱导主要凋亡反应的时间和剂量依赖性的方式，并产生不同的毒理学档案和未分化和分化的神经细胞的反应。发现Au NPs抑制HepG 2细胞的增殖和细胞内钙释放。
Recent advances and progress in nanobiotechnology have demonstrated many nanoparticles (NPs) as potential and novel drug delivery vehicles, therapeutic agents, and contrast agents and luminescent biological labels for bioimaging. The emergence of new biomedical applications based on NPs signifies the need to understand, compare, and manage their cytotoxicity. In this study, we demonstrated the use of high-content screening assay (HCA) as a universal tool to probe the cytotoxicity of NPs and specifically cadmium telluride quantum dots (CdTe QDs) and gold NPs (Au NPs) in NG108-15 murine neuroblastoma cells and HepG2 human hepatocellular carcinoma cells. Neural cells represent special interest for NP-induced cytotoxicity because the optical and electrical functionalities of materials necessary for neural imaging and interfacing are matched well with the properties of many NPs. In addition, the cellular morphology of neurons is particularly suitable for automated high content screening. HepG2 cells represent a good model for high content screening studies since they are commonly used as a surrogate for human hepatocytes in pharmaceutical studies. We found the CdTe QDs to induce primarily apoptotic response in a time- and dosage-dependent manner and produce different toxicological profiles and responses in undifferentiated and differentiated neural cells. Au NPs were found to inhibit the proliferation and intracellular calcium release of HepG2 cells.