Probiotic interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the opportunistic fungal pathogen Candida albicans.
Probiotic interference of Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 with the opportunistic fungal pathogen Candida albicans.
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DOI：
10.1155/2012/636474
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发表时间：
2012
影响因子：
--
通讯作者：
Reid G
中科院分区：
文献类型：
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作者：
Köhler GA;Assefa S;Reid G
白色念珠菌是引起外阴阴道念珠菌病（VVC）的最重要的念珠菌属。VVC对妇女的健康和福祉具有重大的医疗和经济影响。虽然目前的抗真菌治疗是合理有效的，支持和预防措施，如应用益生菌，以减少VVC的发病率。我们研究了益生菌鼠李糖乳杆菌GR-1和罗伊氏乳杆菌RC-14对C.白色念珠菌体外实验表明，乳酸在低pH下在抑制真菌生长方面起主要作用。与乳酸杆菌共培养后的活力染色显示，C。白色念珠菌细胞失去代谢活性并最终被杀死。转录组分析显示应激相关基因表达增加，氟康唑耐药相关基因表达降低，这可能解释了先前联合益生菌治疗临床研究中念珠菌根除率增加的原因。我们的研究结果为益生菌对C.白色念珠菌存活率。
Candida albicans is the most important Candida species causing vulvovaginal candidiasis (VVC). VVC has significant medical and economical impact on women's health and wellbeing. While current antifungal treatment is reasonably effective, supportive and preventive measures such as application of probiotics are required to reduce the incidence of VVC. We investigated the potential of the probiotics Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 towards control of C. albicans. In vitro experiments demonstrated that lactic acid at low pH plays a major role in suppressing fungal growth. Viability staining following cocultures with lactobacilli revealed that C. albicans cells lost metabolic activity and eventually were killed. Transcriptome analyses showed increased expression of stress-related genes and lower expression of genes involved in fluconazole resistance, which might explain the increased eradication of Candida in a previous clinical study on conjoint probiotic therapy. Our results provide insights on the impact of probiotics on C. albicans survival.