Speciation of iodo amino acids by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection
Speciation of iodo amino acids by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection
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DOI：
10.1021/ac00054a018
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发表时间：
1993-03
影响因子：
7.4
通讯作者：
K. Takatera;Tadashi Watanabe
中科院分区：
文献类型：
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作者：
K. Takatera;Tadashi Watanabe
高效液相色谱 (HPLC) 与在线电感耦合等离子体质谱 (ICP/MS) 联用，可对甲状腺球蛋白中常见的碘离子 I - 和五种碘氨基酸（一碘酪氨酸、二碘酪氨酸、3,3',5- 和 3,3',5'-三碘甲状腺原氨酸以及甲状腺素）进行形态分析。绝对检测限范围为碘 35 至 130 pg：这比使用稳定碘同位素的传统方法低大约 1 个数量级
High-performance liquid chromatography (HPLC) has been coupled with on-line inductively coupled plasma mass spectrometry (ICP/MS) for the speciation of iodide ion I - and five iodo amino acids (monoiodotyrosine, diiodotyrosine, 3,3',5- and 3,3',5'-triiodothyronine, and thyroxine) commonly found in thyroglobulin. The absolute detection limits were in a range from 35 to 130 pg as iodine: this is roughly 1 order of magnitude lower than those in conventional methods using stable isotope of iodine