Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae).
Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae).
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硬蜱皮肤蜱（螨类：硬蜱科）抗原蛋白的筛选和鉴定
DOI：
10.3347/kjp.2015.53.6.789
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Hu Y
中科院分区：
文献类型：
--
作者：
Zhang T;Cui X;Zhang J;Wang H;Wu M;Zeng H;Cao Y;Liu J;Hu Y
为了探索蜱蛋白作为进一步开发蜱疫苗的潜在靶点，用抗D筛选了未喂食的雌性森林革蜱的总蛋白。从兔子中提取的 silvarum 血清。 Western blot结果显示，多克隆抗体检测到了约100、68和52 kDa的3个抗原蛋白，这意味着它们可能具有免疫原性。然后，通过 12% SDS-PAGE 分离未喂食的雌性蜱蛋白，并分别通过 LC-MS/MS 切割和分析目标蛋白（100、68 和 52 kDa）。肽序列比较结果表明，它们可能分别是卵黄蛋白原(Vg)、热休克蛋白60(Hsp60)和果糖-1,6-二磷酸醛缩酶(FBA)。这些数据将为进一步验证抗原蛋白以预防 D. silvarum 感染和传播疾病奠定基础。
In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
DOI：
10.3347/kjp.2014.52.1.93
发表时间：
2014-02
期刊：
The Korean journal of parasitology
影响因子：
--
作者：
Hu Y;Zeng H;Zhang J;Wang D;Li D;Zhang T;Yang S;Liu J
通讯作者：
Liu J