Regulation of podocalyxin trafficking by Rab small GTPases in epithelial cells.
Regulation of podocalyxin trafficking by Rab small GTPases in epithelial cells.
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上皮细胞中 Rab 小 GTP 酶对足萼蛋白运输的调节。
DOI：
10.1080/21541248.2016.1211068
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Mrozowska;P. S. and Fukuda;M.
MDCK II 细胞极性建立的特征是顶端糖蛋白足萼蛋白 (PCX) 从外质膜到新形成的顶端结构域的转胞吞作用。这种转胞吞事件由多个步骤组成，包括从质膜内化、通过早期核内体和 Rab11 阳性回收核内体转运，以及递送至顶膜。已知这些步骤由 Rab 小 GTP 酶紧密协调，Rab 小 GTP 酶充当在活性 GTP 结合状态和非活性 GDP 结合状态之间循环的分子开关。然而，我们对哪些 Rab 组调节 PCX 贩运的特定步骤的了解相当有限。最近，我们对二维 (2D) 和三维 (3D) MDCK II 细胞培养物极性建立过程中 Rab GTPase 在 PCX 转胞吞途径中的参与进行了全面分析。在本评论中，我们总结了我们的发现，并将其置于之前报告的背景下。
The characteristic feature of polarity establishment in MDCK II cells is transcytosis of apical glycoprotein podocalyxin (PCX) from the outer plasma membrane to the newly formed apical domain. This transcytotic event consists of multiple steps, including internalization from the plasma membrane, transport through early endosomes and Rab11-positive recycling endosomes, and delivery to the apical membrane. These steps are known to be tightly coordinated by Rab small GTPases, which act as molecular switches cycling between active GTP-bound and inactive GDP-bound states. However, our knowledge regarding which sets of Rabs regulate particular steps of PCX trafficking was rather limited. Recently, we have performed a comprehensive analysis of Rab GTPase engagement in the transcytotic pathway of PCX during polarity establishment in 2-dimensional (2D) and 3-dimensional (3D) MDCK II cell cultures. In this Commentary we summarize our findings and set them in the context of previous reports.