Visualization of Immune Responses in the Cornea.
Visualization of Immune Responses in the Cornea.
复制标题
角膜免疫反应的可视化。
DOI：
10.1097/ico.0000000000001354
复制
发表时间：
2017
期刊：
影响因子：
2.8
通讯作者：
Perez,VictorL
中科院分区：
文献类型：
--
作者：
Perez,VictorL
眼睛已成为研究和了解局部和全身免疫反应的有用部位。角膜的易接近性和透明性允许眼睛结构、血管和淋巴管的直接可视化，从而允许真实的实时跟踪正常和病理生物过程。作为免疫系统的窗口，我们已经使用眼睛来剖析角膜炎症反应的机制，包括先天性和适应性免疫反应。我们已经确定，眼部微环境通过局部产生选定的趋化因子，将不同的炎性细胞群募集到角膜来调节这些免疫反应。此外，T细胞和巨噬细胞之间的串扰是对角膜同种异体抗原的眼部免疫应答发展中的常见且关键的步骤。这篇综述总结了我们小组使用活体荧光共聚焦显微镜捕获先天性和适应性角膜免疫反应的克里思，幅度和功能所产生的数据。
The eye has become a useful site for the investigation and understanding of local and systemic immune responses. The ease of access and transparency of the cornea permits direct visualization of ocular structures, blood vessels, and lymphatic vessels, allowing for the tracking of normal and pathological biological processes in real time. As a window to the immune system, we have used the eye to dissect the mechanisms of corneal inflammatory reactions that include innate and adaptive immune responses. We have identified that the ocular microenvironment regulates these immune responses by recruiting different populations of inflammatory cells to the cornea through local production of selected chemokines. Moreover, crosstalk between T cells and macrophages is a common and crucial step in the development of ocular immune responses to corneal alloantigens. This review summarizes the data generated by our group using intravital fluorescent confocal microscopy to capture the tempo, magnitude, and function of innate and adaptive corneal immune responses.