Effect of cell cycle stages on the central density of Enterococcus faecium ATCC 9790.
Effect of cell cycle stages on the central density of Enterococcus faecium ATCC 9790.
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细胞周期阶段对屎肠球菌 ATCC 9790 中心密度的影响。
DOI：
10.1128/jb.171.4.1982-1986.1989
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发表时间：
1989
影响因子：
3.2
通讯作者：
Daneo-Moore,L
中科院分区：
文献类型：
--
作者：
Bourbeau,P;Dicker,D;Higgins,ML;Daneo-Moore,L
利用残余分裂法和形态学分析方法，研究了以不同速率生长的粪肠球菌的细胞分裂周期事件的时间。这两种方法给出的D1（染色体复制完成）和D2（分隔完成）的起始时间基本相同。测定了在不同生长速率下牛血清白蛋白中表现出相位反转中心的细胞频率。该数据符合这样一个模型，即快速生长的细胞在参与正在进行的分裂的染色体复制周期完成时折射率（假定代表中心密度）增加，而缓慢生长的培养物在完成分离时中心密度增加。中心密度增加的时间与新的表面生长位点的起始时间之间没有相关性。
Cultures of Enterococcus faecium growing at various rates were examined for timing of cell division cycle events by using the method of residual divisions and a morphological analysis. Both methods gave essentially the same timing for the onset of D1 (completion of chromosome replication) and of D2 (completion of septation). Frequencies of cells exhibiting a phase-reversed center in bovine serum albumin at various growth rates were determined. The data fit a model in which rapidly growing cells increase in refractive index (which is assumed to represent central density) at completion of the chromosome replication cycle involved in the ongoing division, whereas slowly growing cultures increase in central density at the time of completion of septation. There was no correlation between the timing of increase in central density and the timing of initiation of new sites of surface growth.