Epidermal growth factor stimulates mouse placental lactogen I but inhibits mouse placental lactogen II secretion in vitro.
Epidermal growth factor stimulates mouse placental lactogen I but inhibits mouse placental lactogen II secretion in vitro.
复制标题
表皮生长因子在体外刺激小鼠胎盘催乳素 I，但抑制小鼠胎盘催乳素 II 分泌。
DOI：
10.1073/pnas.89.23.11396
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发表时间：
1992
影响因子：
11.1
通讯作者：
Talamantes,F
中科院分区：
文献类型：
--
作者：
Yamaguchi,M;Ogren,L;Endo,H;Thordarson,G;Kensinger,R;Talamantes,F
本研究旨在确定表皮生长因子（EGF）是否调节小鼠胎盘催乳素（mPL）-I和mPL-II的分泌。从妊娠第7、9和11天的胎盘制备原代细胞培养物，并培养长达5天。在培养基中加入EGF（20 ng/ml），在妊娠第7天和第9天的细胞中，在培养的第二天显著刺激mPL-I分泌，在第7、9和11天的细胞中，在培养的第三天或第四天显著抑制mPL-II分泌。用妊娠第7天的细胞进行的剂量反应研究表明，刺激mPL-I分泌和抑制mPL-II分泌的EGF的最小浓度为1.0 ng/ml。EGF不影响细胞的DNA含量或细胞活力，通过台盼蓝排除法评估，也没有对蛋白质合成的一般影响。在小鼠胎盘细胞培养物中有三种类型的含PL的巨细胞：含有mPL-I或mPL-II的细胞和含有两种激素的细胞。免疫细胞化学分析和反向溶血空斑试验表明，EGF治疗伴随着产生mPL-I的细胞数量的显着增加，但PL细胞中含有mPL-I，有没有变化的细胞的分数，只含有mPL-I或分数，包含mPL-I和mPL-II。相反，EGF处理影响PL细胞中mPL-II的分布。在对照培养物中，约75%含有mPL-II的细胞也含有mPL-I，但在EGF处理的培养物中，所有含有mPL-II的细胞也含有mPL-I。这些数据表明，EGF调节mPL-I和mPL-II分泌至少部分通过调节PL细胞分化。
This study was undertaken to determine whether epidermal growth factor (EGF) regulates the secretion of mouse placental lactogen (mPL)-I and mPL-II. Primary cell cultures were prepared from placentas from days 7, 9, and 11 of pregnancy and cultured for up to 5 days. Addition of EGF (20 ng/ml) to the medium resulted in significant stimulation of mPL-I secretion by the second day of culture in cells from days 7 and 9 of pregnancy and significant inhibition of mPL-II secretion by the third or fourth day of culture in cells from days 7, 9, and 11. Dose-response studies carried out with cells from day 7 of pregnancy demonstrated that the minimum concentration of EGF that stimulated mPL-I secretion and inhibited mPL-II secretion was 1.0 ng/ml. EGF did not affect the DNA content of the cells or cell viability, assessed by trypan blue exclusion, nor did it have a general effect on protein synthesis. There are three types of PL-containing giant cells in mouse placental cell cultures: cells that contain either mPL-I or mPL-II and cells that contain both hormones. Immunocytochemical analysis and the reverse hemolytic plaque assay indicated that EGF treatment was accompanied by a significant increase in the number of cells that produce mPL-I, but among the PL cells that contained mPL-I, there was no change in the fraction of cells that contained only mPL-I or the fraction that contained both mPL-I and mPL-II. In contrast, EGF treatment did affect the distribution of mPL-II among PL cells. In control cultures, about 75% of the cells that contained mPL-II also contained mPL-I, but in EGF-treated cultures, all of the cells that contained mPL-II also contained mPL-I. These data suggest that EGF regulates mPL-I and mPL-II secretion at least partly by regulating PL cell differentiation.