Regional variation of biochemical characteristics and antigeneity in Great Basin rattlesnake (Crotalus viridis lutosus) venom.
Regional variation of biochemical characteristics and antigeneity in Great Basin rattlesnake (Crotalus viridis lutosus) venom.
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大盆地响尾蛇（Crotalus viridis lutosus）毒液生化特征和抗原性的区域差异。
DOI：
10.1016/0305-0491(90)90184-u
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Straight,RC
中科院分区：
文献类型：
--
作者：
Adame,BL;Soto,JG;Secraw,DJ;Perez,JC;Glenn,JL;Straight,RC
1. 采用高效液相色谱-阴离子交换法对美国犹他州和亚利桑那州不同地区的绿爪蟾（Crotalus viridis lutosus） 3份集体和20份个体毒液进行了筛选和分离。2. 用ELISA法检测混合毒液样品和组分的出血、胶原酶和磷脂酶活性，以及对一种单克隆抗体（CA-P-8）的反应性。3. 根据HPLC图谱将20个样品分为4组。4. ELISA结果和毒液样品的特异性出血活性显示，尽管来自同一物种，但在纬度分布上存在差异。
1. Three pooled and 20 individual venom samples of Crotalus viridis lutosus from different localities in Utah and Arizona were screened and fractionated with HPLC-anion exchange. 2. Pooled venom samples and fractions were tested for hemorrhagic, collagenase, and phospholipase activities, and reactivity to a monoclonal antibody against a hemorrhagin from C. atrox venom (CA-P-8) using ELISA. 3. The 20 individual samples were organized into four groups based on their HPLC profiles. 4. ELISA results and specific hemorrhagic activity of the venom samples displayed a variation in latitidinal distribution although from the same species.