Ciliary neurotrophic factor is expressed in the magnocellular neurosecretory system of the rat in vivo: evidence for injury- and activity-induced upregulation.
Ciliary neurotrophic factor is expressed in the magnocellular neurosecretory system of the rat in vivo: evidence for injury- and activity-induced upregulation.
复制标题
睫状神经营养因子在大鼠体内的大细胞神经分泌系统中表达：损伤和活动诱导上调的证据。
DOI：
10.1016/j.expneurol.2005.09.009
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Paden,CharlesM
中科院分区：
文献类型：
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作者：
Watt,JohnA;Bone,Sven;Pressler,Mandy;Cranston,HarwoodJ;Paden,CharlesM
虽然纤毛神经营养因子（CNTF）已被证明可以促进体外大细胞神经元的存活，当外源性应用于室旁核和视上核（SON）外植体时，其在体内损伤后成人大细胞神经分泌系统（MNS）中的表达或调节知之甚少。因此，我们利用原位杂交和免疫细胞化学分析结合定量光学密度分析来确定CNTF的细胞来源，并在成年大鼠的下丘脑-神经垂体束单侧横断后检查其表达的时间模式。在完整的大鼠中，CNTF免疫反应性（CNTF-ir）主要局限于位于SON下的腹侧神经胶质区内已识别的星形胶质细胞。定量光学密度分析表明，损伤后3 - 30天，CNTF-ir的比例面积显著升高。在未受伤的对侧SON中也观察到显著但更有限的增加。原位杂交证实了CNTF在axoized SON中的表达和上调。这些结果证明了CNTF在成年啮齿动物MNS中的体内表达，并提供了CNTF水平在损伤和萌发SON中分别对直接损伤和代谢活性升高作出反应的证据。
Although ciliary neurotrophic factor (CNTF) has been shown to promote the survival of magnocellular neurons when applied exogenously to explants of the paraventricular and supraoptic nuclei (SON) in vitro, little is known regarding its expression or regulation in the adult magnocellular neurosecretory system (MNS) following injury in vivo. Therefore, we utilized in situ hybridization and immunocytochemical analysis in conjunction with quantitative optical densitometric analysis to identify the cellular source of CNTF and examine the temporal pattern of its expression, following unilateral transection of the hypothalamo-neurohypophysial tract in the adult rat. In intact rats, CNTF immunoreactivity (CNTF-ir) was predominantly localized within identified astrocytes within the ventral glial limitans subjacent to the SON. Quantitative optical densitometric analysis of CNTF-ir levels in the axotomized SON demonstrated that the proportional area of CNTF-ir was significantly elevated between 3 and 30 days following injury. A significant but more limited increase was also observed in the non-injured contralateral SON. In situ hybridization confirmed the expression and upregulation of CNTF in the axotomized SON. These results demonstrate the expression of CNTF in the adult rodent MNS in vivo and provide evidence that levels of CNTF are upregulated in response to both direct injury, and heightened metabolic activity, within the lesioned and sprouting SON, respectively.