INTRAVITREAL CHEMOTACTIC AND MITOGENIC ACTIVITY - IMPLICATION OF BLOOD-RETINAL BARRIER BREAKDOWN
INTRAVITREAL CHEMOTACTIC AND MITOGENIC ACTIVITY - IMPLICATION OF BLOOD-RETINAL BARRIER BREAKDOWN
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DOI：
10.1001/archopht.1986.01050230123046
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发表时间：
1986-11-01
影响因子：
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通讯作者：
JACCOMA, EH
中科院分区：
文献类型：
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作者：
CAMPOCHIARO, PA;BRYAN, JA;JACCOMA, EH
我们通过几种不同的机制破坏色素兔子的血视网膜屏障（BRB）：视网膜冷冻固定、视网膜激光、静脉注射碘酸钠（30 mg/kg）或玻璃体内注射肾上腺素（0.1 mL，10-3 M）。 BRB 分解程度通过计算机玻璃体荧光光度法监测。治疗后72小时，处死兔子，迅速取出眼睛，清洗，放入液氮中。然后小心地分离玻璃体，使其不受其他眼组织的污染，均质化并等分。对于每个样品，我们测量了蛋白质含量、视网膜色素上皮 (RPE) 细胞趋化活性和 RPE 促有丝分裂活性。通过玻璃体荧光光度法和玻璃体内蛋白质浓度测量，上述每种方式都会导致 BRB 显着分解，而玻璃体平坦部注射盐水 (0.1 mL) 则不会。用上述每种方式治疗的眼睛的玻璃体引起了 RPE 迁移的显着刺激（冷冻固定，684%；激光，211%；碘酸钠，480%；玻璃体内肾上腺素，546%），而对照玻璃体和注射盐水的玻璃体分别仅引起 89% 和 77% 的刺激。同样，用上述方式治疗的眼睛的玻璃体引起了 RPE 增殖的显着刺激（116% 至 159%），而对照玻璃体仅导致比基线增加 56%。增殖性玻璃体视网膜病变是一种细胞迁移和增殖发挥重要作用的疾病过程，最常见于手术干预后。由于血清来源的化学引诱剂和有丝分裂原在玻璃体内积聚，手术时 BRB 的分解可能是一个关键事件。
We produced breakdown of the blood-retinal barrier (BRB) in pigmented rabbits by several different mechanisms: retinal cryopexy, retinal laser, intravenous sodium iodate (30 mg/kg), or intravitreal injection of epinephrine (0.1 mL, 10-3 M). The degree of BRB breakdown was monitored by computerized vitreous fluorophotometry. At 72 hours after treatment, rabbits were killed, and eyes were quickly removed, washed, and placed in liquid nitrogen. The vitreous was then carefully isolated free of contamination from other ocular tissues, homogenized, and aliquotted. For each sample we measured protein content, retinal pigment epithelial (RPE) cell chemotactic activity, and RPE mitogenic activity. Each of the above modalities produced significant breakdown of the BRB as measured by vitreous fluorophotometry and intravitreal protein concentration, while a pars plana intravitreal injection of saline (0.1 mL) did not. Vitreous from eyes treated with each of the above modalities caused significant stimulation of RPE migration (cryopexy, 684%; laser, 211%; sodium iodate, 480%; intravitreal epinephrine, 546%), while control vitreous and saline-injected vitreous caused only 89% and 77% stimulations, respectively. Similarly, vitreous from eyes treated with the above modalities caused significant stimulation of RPE proliferation (116% to 159%), while control vitreous caused only a 56% increase above baseline. Proliferative vitreoretinopathy, a disease process in which cellular migration and proliferation play important roles, occurs most commonly after surgical intervention. Breakdown of the BRB at the time of surgery may be a critical event due to intravitreal accumulation of serum-derived chemoattractants and mitogens.