Genetic control of cellular morphogenesis in Muller glia
Genetic control of cellular morphogenesis in Muller glia
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DOI：
10.1002/glia.23615
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发表时间：
2019-07-01
期刊：
影响因子：
6.2
通讯作者：
Harris, William A.
中科院分区：
文献类型：
--
作者：
Charlton-Perkins, Mark;Almeida, Alexandra D.;Harris, William A.
细胞的形状对于身体中每个组织中每个细胞的正常功能至关重要。对于中枢神经系统中形态高度多样的神经细胞和胶质细胞来说尤其如此。这些细胞或实际上任何细胞获得其特定细胞形态的分子过程在很大程度上仍未被探索。为了鉴定参与脊椎动物视网膜中主要胶质细胞类型Muller glia （MG）形态发生的基因，我们在斑马鱼（Danio rerio）中使用了基因组和CRISPR技术。我们确定了41个基因参与MG细胞形态发生的各个方面，并揭示了解剖特征添加的顺序步骤与调节这些步骤的功能相关基因的表达之间的惊人一致性。我们注意到许多在斑马鱼MG中优先表达的基因在不同物种的胶质细胞中表现出保守性，这表明在进化上保守的胶质细胞发育途径。
Cell shape is critical for the proper function of every cell in every tissue in the body. This is especially true for the highly morphologically diverse neural and glia cells of the central nervous system. The molecular processes by which these, or indeed any, cells gain their particular cell-specific morphology remain largely unexplored. To identify the genes involved in the morphogenesis of the principal glial cell type in the vertebrate retina, the Muller glia (MG), we used genomic and CRISPR based strategies in zebrafish (Danio rerio). We identified 41 genes involved in various aspects of MG cell morphogenesis and revealed a striking concordance between the sequential steps of anatomical feature addition and the expression of cohorts of functionally related genes that regulate these steps. We noted that the many of the genes preferentially expressed in zebrafish MG showed conservation in glia across species suggesting evolutionarily conserved glial developmental pathways.