Peptide Arrays with a Chip
Peptide Arrays with a Chip
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DOI：
10.1007/978-1-60761-845-4_9
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发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Breitling, Frank
中科院分区：
文献类型：
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作者：
Nesterov, Alexander;Doersam, Edgar;Breitling, Frank
如今，光刻方法可实现> 50,000个寡核苷酸的组合合成，每厘米（2），这是一种彻底改变了基因组学领域的进步。预计蛋白质组学领域也有类似的发展，只要可以使用价格合理的非常高密度的肽阵列。但是，肽阵列滞后于寡核苷酸阵列。这主要是由于与光刻相关的20种不同氨基酸的单体重复连续耦合，这加起来多达数量过多的耦合周期。基于电荷固体氨基酸颗粒的组合合成可以解决此问题。计算机芯片连续地解决不同的电荷颗粒以获得固体支撑，在完成后，整个固体氨基酸颗粒的整层都会一次融化。迄今为止，这种释放氨基酸将氨基酸固定在一个单一耦合反应中，将所有20种不同的氨基酸融入了载体。该方法应允许将整个基因组转化为一组重叠的肽，用于蛋白质组研究。
Today, lithographic methods enable combinatorial synthesis of >50,000 oligonucleotides per cm(2), an advance that has revolutionized the whole field of genomics. A similar development is expected for the field of proteomics, provided that affordable, very high-density peptide arrays are available. However, peptide arrays lag behind oligonucleotide arrays. This is mainly due to the monomer-by-monomer repeated consecutive coupling of 20 different amino acids associated with lithography, which adds up to an excessive number of coupling cycles. A combinatorial synthesis based on electrically charged solid amino acid particles resolves this problem. A computer chip consecutively addresses the different charged particles to a solid support, where, when completed, the whole layer of solid amino acid particles is melted at once. This frees hitherto immobilized amino acids to couple all 20 different amino acids in one single coupling reaction to the support. The method should allow for the translation of entire genomes into a set of overlapping peptides to be used in proteome research.