Internal eliminated sequences are removed prior to chromosome fragmentation during development in Euplotes crassus.
Internal eliminated sequences are removed prior to chromosome fragmentation during development in Euplotes crassus.
复制标题
在粗糙游标的发育过程中，内部消除的序列在染色体断裂之前被去除。
DOI：
10.1093/nar/18.4.845
复制
发表时间：
1990
影响因子：
14.9
通讯作者：
Klobutcher,LA
中科院分区：
文献类型：
--
作者：
Tausta,SL;Klobutcher,LA
多毛类纤毛虫在性周期结束后会经历一个大规模的基因组重排过程。一种常见的重排类型是从DNA分子的内部区域去除DNA序列（内部消除序列; IES）。在这项研究中，我们的特点是去除IESinEuplotes crassus。Southern杂交分析结合细胞学观察表明，IES去除是在大核发育的早期事件，发生在多线染色体阶段和之前的染色体断裂过程。结果与IES去除通过分子内DNA断裂和重新连接过程发生一致。
The hypotrichous ciliated protozoa undergo a massive genome rearrangement process after their sexual cycle. One frequent type of rearrangement is the removal of DNA sequences (internal eliminated sequences; IESs) from internal regions of DNA molecules. In this study, we characterized the removal of IESs inEuplotes crassus. Southern hybridization analyses combined with cytological observations Indicated that IES removal is an early event in macronuclear development, occurring during the polytene chromosome stage and prior to the chromosome fragmentation process. The results are consistent with IES removal occurring via an intramolecular DNA breakage and rejoining process.