PLEUROPNEUMONIA-LIKE (MYCOPLASMA) INFECTIONS OF TISSUE CULTURE
PLEUROPNEUMONIA-LIKE (MYCOPLASMA) INFECTIONS OF TISSUE CULTURE
复制标题
组织培养的胸膜肺炎样（支原体）感染
DOI：
10.1128/jb.81.4.626-635.1961
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发表时间：
1961
影响因子：
3.2
通讯作者：
C. Shepard
中科院分区：
文献类型：
--
作者：
T. Carski;C. Shepard
The frequent contamination of animal cell lines by pleuropneumonia-like organisms (PPLO) reported by Robinson, Wichelhausen, and Roizman (1956) and by Collier (1957) is of obvious consequence to research workers using tissue culture systems. The presence of these microorganisms could influence results obtained in diverse studies, for example, of nutritive requirements of the cells or of viral antigens grown in them. The cultural methods commonly employed for the demonstration of PPLO have been somewhat complicated, but they are not difficult with the medium of Barile, Yaguchi, and Eveland (1958). To gain a clearer comprehension of the problem we have studied several aspects of the PPLO contamination of tissue cultures. An interesting model of host-parasite relationships emerges. Growth of PPLO occurs only in the presence of tissue culture cells, which are able to support growth of PPLO to high concentration without manifesting important cytopathic changes. It seems clear that unwanted PPLO infections of cell lines need occasion little future difficulties since it is possible to render the cell lines free of PPLO with antibiotic treatment. Artificial media for the growth of PPLO from tissue cultures. A) The medium of Barile, Yaguchi, and Eveland (hereafter called medium I) consists of 3.7% commercial brain heart infusion, 0.2% powdered yeast extract, 1.3% agar, and 15% outdated human bank blood (which contains citrate-glucose solution). In 3 to 4 days this medium grows distinct colonies that may be easily counted and may be transferred by simple streaking with a bacteriological loop. Modifications in the medium were tested by plating out 0.01 ml of 10-fold dilutions of tissue culture fluid of a HeLa cell line, and observations were made daily of the size and number of colonies. Usually 1 to 20 colonies were seen with
