Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping
Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping
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DOI：
10.1021/jf902455j
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发表时间：
2009-10-28
影响因子：
6.1
通讯作者：
Greenland, Andy
中科院分区：
文献类型：
--
作者：
Lee, David;La Mura, Maurizio;Greenland, Andy
DNA提取是高通量基因分型的主要成本。环介导等温扩增（LAMP）检测已被用于检测两个转基因（GM）相关序列。从用最小的制备处理（在水中破坏）制备的叶和玉米种子组织扩增靶DNA序列证明了LAMP在通常抑制PCR的条件下工作的能力。在这些样品中的宽动态检测范围表明，即使当靶以这样的粗制形式存在时，LAMP也是高度敏感的。LAMP提供了一种降低基因分型成本以及简化测试程序的方法。
DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures.