Immunologic profile of juvenile periodontitis. II. Neutrophil chemotaxis, phagocytosis and spore germination.
Immunologic profile of juvenile periodontitis. II. Neutrophil chemotaxis, phagocytosis and spore germination.
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青少年牙周炎的免疫学特征。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
Robert K. Nauman
中科院分区：
文献类型：
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作者：
Jon B. Suzuki;Jon B. Suzuki;Jon B. Suzuki;B. Collison;W. Falkler;Robert K. Nauman
青少年牙周炎（JP）患者分为两种公认的疾病形式之一：影响门牙和第一磨牙的局部形式（LJP）和影响超过14颗牙齿的全身形式（GJP）。中性粒细胞在LJP的病因和发病机制中的作用最近已被认识。进行了旨在确认先前与LJP中中性粒细胞趋化性缺陷相关的信息的实验。还完成了旨在证明细菌孢子吞噬作用和诱导孢子萌发的显著差异的其他实验。年龄和性别匹配的健康受试者用作“内部”对照（实验同时进行）。对29例LJP、24例GJP和24例健康受试者的外周血中性粒细胞进行了趋化性评估。该试验在Boyden扩散室中进行，使用10（-8）M N-甲酰甲硫氨酰亮氨酰苯丙氨酸作为化学引诱物。趋化缺陷定义为低于健康受试者平均值2个标准差。29例LJP中的23例（79%）和25例GJP中的14例（58%）有中性粒细胞缺陷。使用放射性标记的细菌孢子[45 Ca]蜡样芽孢杆菌在体外进行外周血中性粒细胞吞噬试验。29例LJP中有18例（62%）和24例GJP中有7例（29%）存在吞噬缺陷。中性粒细胞诱导的孢子萌发在体外评估表明，13的20个LJP（65%）和3的8个GJP（38%）有这个缺陷。这些观察结果表明，JP患者有异常的中性粒细胞的趋化性，吞噬功能和孢子萌发。
Patients with juvenile periodontitis (JP) were grouped into one of the two recognized forms of the disease: a localized form affecting incisors and first molars (LJP) and a generalized form affecting more than 14 teeth (GJP). The role of the neutrophil in the etiology and pathogenesis of LJP has recently been recognized. Experiments aimed at confirming previous information related to neutrophil chemotaxis defects in LJP were performed. Additional experiments aimed at demonstrating significant differences in phagocytosis of bacterial spores and inducing spore germination were also completed. Age- and sex-matched healthy subjects were used as "internal" controls (experiments run concomitantly). Peripheral blood neutrophils from 29 LJP, 24 GJP and 24 healthy subjects were assessed for chemotaxis. This assay was performed in a Boyden diffusion chamber using 10(-8) M N-formylmethionylleucylphenylalanine as chemoattractant. A chemotactic defect was defined being 2 standard deviations below the mean of healthy subjects. Twenty-three of 29 LJP (79%) and 14 of 25 GJP (58%) had a neutrophil defect. Peripheral blood neutrophil phagocytosis assays were performed in vitro using radiolabeled bacterial spores [45Ca]Bacillus cereus. Eighteen of 29 LJP (62%) and 7 of 24 GJP (29%) had a phagocytic defect. Neutrophil-induced spore germination assessed in vitro showed that 13 of 20 LJP (65%) and 3 of 8 GJP (38%) had this defect. These observations indicate that patients with JP have abnormalities in neutrophil functions of chemotaxis, phagocytosis and spore germination.