SWI3B and HDA6 interact and are required for transposon silencing in Arabidopsis
SWI3B and HDA6 interact and are required for transposon silencing in Arabidopsis
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SWI3B 和 HDA6 相互作用并且是拟南芥中转座子沉默所必需的
DOI：
10.1111/tpj.14666
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发表时间：
2020
期刊：
影响因子：
7.2
通讯作者：
Liu Xuncheng
中科院分区：
文献类型：
--
作者：
Yang Jie;Yuan Lianyu;Yen Ming-Ren;Zheng Feng;Ji Rujun;Peng Tao;Gu Dachuan;Yang Songguang;Cui Yuhai;Chen Pao-Yang;Wu Keqiang;Liu Xuncheng
虽然共价组蛋白乙酰化/去乙酰化和ATP依赖性染色质重塑的相互作用对真核生物染色质结构和基因表达的调节至关重要，但植物中潜在的分子机制仍不清楚。本研究显示，拟南芥sw3b （SWI/SNF染色质重塑复合体的一个重要亚基）与RPD3/HDA1型组蛋白去乙酰化酶HDA6在体内和体外均存在直接相互作用。此外，SWI3B和HDA6共同抑制转座子子集的转录。SWI3B和HDA6都通过降低组蛋白H3赖氨酸9乙酰化，但增加组蛋白H3赖氨酸9二甲基化、DNA甲基化和核小体占用来维持转座子沉默。我们的研究结果表明，在拟南芥中，SWI3B和HDA6可能在相同的协同抑制复合体中起作用，以维持转座子沉默。
Although the interplay of covalent histone acetylation/deacetylation and ATP‐dependent chromatin remodelling is crucial for the regulation of chromatin structure and gene expression in eukaryotes, the underlying molecular mechanism in plants remains largely unclear. Here we show a direct interaction between Arabidopsis SWI3B, an essential subunit of the SWI/SNF chromatin‐remodelling complex, and the RPD3/HDA1‐type histone deacetylase HDA6 bothinvitroandin vivo. Furthermore, SWI3B and HDA6 co‐repress the transcription of a subset of transposons. Both SWI3B and HDA6 maintain transposon silencing by decreasing histone H3 lysine 9 acetylation, but increasing histone H3 lysine 9 di‐methylation, DNA methylation and nucleosome occupancy. Our findings reveal that SWI3B and HDA6 may act in the same co‐repressor complex to maintain transposon silencing in Arabidopsis.