Neutrophils infiltrating pancreatic ductal adenocarcinoma indicate higher malignancy and worse prognosis
Neutrophils infiltrating pancreatic ductal adenocarcinoma indicate higher malignancy and worse prognosis
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DOI：
10.1016/j.bbrc.2018.05.024
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发表时间：
2018-06-18
影响因子：
3.1
通讯作者：
Cui, Yunfu
中科院分区：
文献类型：
--
作者：
Wang, Yufu;Fang, Tianyi;Cui, Yunfu
CD177被认为代表中性粒细胞。我们通过R2平台（http：//www.example.com）分析了CD177的mRNA表达水平和PDAC的临床随访调查，以估计来自基因表达综合（GEO）数据集（GSE 21501，包含来自102名PDAC患者的样本）的总生存期（OS）。r2.amc.nl我们还通过基因本体论（GO）和京都基因和基因组百科全书（KEGG）分析来分析CD 177的相关基因，以预测中性粒细胞与PDAC预后的潜在关系。然后我们对手术标本进行苏木精和伊红（H&E）染色和免疫组织化学染色，以验证中性粒细胞在PDAC组织中的浸润。在分析GEO数据集（GSE 21501，包含来自102名PDAC患者的样本）中提供的mRNA表达数据和临床随访调查以及23名PDAC患者的临床病理数据后，我们证明CD 177与不良预后相关。单变量Kaplan Meier生存分析显示OS与CD177表达增加呈负相关（P = 0.012）。R2平台进行基因相关分析，磷酸二酯酶4D与CD177表达呈正相关（R = 0.413，P < 0.001）。23例PDAC手术标本的H&E染色和CD177免疫组化显示嗜中性粒细胞在癌细胞周围的间质和血管中积聚。免疫组化染色显示CD177在PDAC瘤组织周围的间质和血管中呈高表达，与H&E染色结果相似。CD177的表达可用于代表中性粒细胞的浸润，这可能在PDAC中具有潜在的预后价值。(C)2018爱思唯尔公司All rights reserved.
CD177 is considered to represent neutrophils. We analyzed mRNA expression level of CD177 and clinical follow-up survey of PDAC to estimate overall survival (OS) from Gene Expression Omnibus (GEO) dataset (GSE21501, containing samples from 102 PDAC patients) by R2 platform (http://r2.amc.nl). We also analyzed correlated genes of CD177 by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to predict the potential relationship between neutrophils and prognosis of PDAC. We then performed hematoxylin and eosin (H&E) staining and immunohistochemical staining of surgical specimens to verify infiltration of neutrophils in PDAC tissues. After analyzing mRNA expression data and clinical follow-up survey provided in the GEO dataset (GSE21501, containing samples from 102 PDAC patients) and clinicopathological data of 23 PDAC patients, we demonstrated that CD177 was correlated with poor prognosis. The univariate Kaplan Meier survival analysis revealed that OS was inversely associated with increased expression of CD177 (P = 0.012). Expression of phosphodiesterase (PDE)4D was positively related to CD177 in gene correlation analysis (R = 0.413, P < 0.001) by R2 platform. H&E staining and immunohistochemistry of CD177 in 23 PDAC surgical samples showed accumulation of neutrophils in the stroma and blood vessels around the cancer cells. In addition, immunohistochemical staining showed that CD177 was highly expressed in the stroma and blood vessels around tumor tissues of PDAC, which was similar to H&E staining. Expression of CD177 can be used to represent infiltration of neutrophils, which may have potential prognostic value in PDAC. (C) 2018 Elsevier Inc. All rights reserved.