Detecting protein function and protein-protein interactions from genome sequences
Detecting protein function and protein-protein interactions from genome sequences
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DOI：
10.1126/science.285.5428.751
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发表时间：
1999-07-30
期刊：
影响因子：
56.9
通讯作者：
Eisenberg, D
中科院分区：
文献类型：
--
作者：
Marcotte, EM;Pellegrini, M;Eisenberg, D
本文提出了一种计算方法，根据观察到某些相互作用的蛋白质对在另一种生物体中具有融合成单个蛋白质链的同源物的基础上，从基因组序列推断蛋白质相互作用。从许多基因组中搜索序列发现，在大肠杆菌中有6809个这样的蛋白质-蛋白质相互作用，在酵母中有45,502个。这些对中的许多成员被证实在功能上相关;计算过滤进一步丰富了相互作用。一些蛋白质与其他几种蛋白质有联系;这些耦合的联系似乎代表了功能性的相互作用，如复合物或途径。实验证实的相互作用对记录在相互作用蛋白质数据库中。
A computational method is proposed for inferring protein interactions from genome sequences on the basis of the observation that some pairs of interacting proteins have homologs in another organism fused into a single protein chain. Searching sequences from many genomes revealed 6809 such putative protein-protein interactions in Escherichia coli and 45,502 in yeast. Many members of these pairs were confirmed as functionally related; computational filtering further enriches for interactions. Some proteins have links to several other proteins; these coupled links appear to represent functional interactions such as complexes or pathways. Experimentally confirmed interacting pairs are documented in a Database of interacting Proteins.