Determination of Δ9-THC in whole blood using gas chromatography-mass spectrometry
Determination of Δ9-THC in whole blood using gas chromatography-mass spectrometry
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DOI：
10.1093/jat/26.8.575
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发表时间：
2002-11-01
影响因子：
2.5
通讯作者：
Drummer, OH
中科院分区：
文献类型：
--
作者：
Chu, MHC;Drummer, OH
建立了一种简便、可靠的液液萃取-气相色谱-质谱法测定全血中Δ9-四氢大麻酚（THC）的方法。该物质在五氟丙醇中用五氟丙酸酐衍生化。对于1 mL样本，检测限为0.5 ng/mL，回收率大于70%。在1、5和25 ng/mL THC浓度下计算的试验内变异系数（CV）为3.1%-5.2%，试验间CV为6.4%-9.5%。准确度在95%到97%之间。详细介绍了萃取和衍生化条件的优化。
A simple and reliable liquid-liquid extraction method for the determination of Δ9-tetrahydrocannabinol (THC) in whole blood utilizing gas chromatography-mass spectrometry in electron impact mode is described. The substance is derivatized with pentafluoropropionic anhydride in pentafluropropanol. The limit of detection is 0.5 ng/mL for a 1-mL specimen, with recovery greater than 70%. The intra-assay coefficient of variation (CV) is 3.1% to 5.2%, and the interassay CV is 6.4% to 9.5%, calculated at THC concentrations of 1, 5, and 25 ng/mL. The accuracy is between 95 and 97%. The optimization of extraction and derivatization conditions is detailed.