Interphase cytogenetics of gastric carcinoma: Fluorescence in situ hybridization (FISH) applied to cells obtained from formalin‐fixed paraffin‐embedded tissues-猫眼课题宝

胃癌的间期细胞遗传学：荧光原位杂交（FISH）应用于福尔马林固定石蜡包埋组织中获得的细胞

DOI：

发表时间：

1995

期刊：

Pathology international (Print)

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作者：

Y. Gomyo;H. Andachi;Katsuhito Nagao;M. Ikeguchi;Hisao Ito

应用α-卫星DNA探针荧光原位杂交（FISH）技术对福尔马林固定和石蜡包埋的胃癌组织进行间期细胞遗传学研究。首先对两种胃癌细胞系TMK-1和MKN-28进行了细胞遗传学分析。在25个TMK-1细胞核型中，18个细胞的7号染色体显示三体性，17号染色体显示二体性。大多数MKN-28细胞显示7号和17号染色体的二体性。从培养的细胞中获得单独分离的TMK-1和MKN-7细胞悬液，并从裸鼠异种移植瘤中获得的用福尔马林固定0、1、3和5天的石蜡包埋组织标本中获得。用核型制备物分析的7号和17号染色体的数量与FISH测定的数量完全一致，即使在石蜡包埋的标本中也是如此。然而，福尔马林固定5天后，标本中无信号显示的肿瘤细胞数量增加。在10例手术切除的胃癌中，石蜡包埋组织细胞中7号和17号染色体的主要信号数为2（二体性），除1例乳头状癌外，其7号染色体为三体。在4例7号染色体和5例17号染色体中发现了显示三体性的大型亚群（超过20%）。高分化癌的三体率高于低分化癌。这些研究结果表明，FISH技术是一个有用的工具，用于检测胃腺癌细胞中的染色体畸变，即使在石蜡包埋标本，只要组织用福尔马林固定适当的时间。

The interphase cytogenetics in formalin‐fixed and paraffin‐embedded gastric cancer tissues were examined by fluorescence in sku hybridization (FISH) with α‐satellite DNA probes. Two gastric carcinoma cell lines, TMK‐1 and MKN‐28, were first analyzed cytogenetically. Of 25 TMK‐1 cell karyotypes, chromosome 7 showed trisomy and chromosome 17 showed disomy in 18 cells. Most MKN‐28 cells showed disomy of both chromosomes 7 and 17. Suspensions of singly isolated TMK‐1 and MKN‐7 cells were obtained from the cultured cells, and from paraffinembedded tissue specimens fixed with formalin for 0, 1, 3 and 5 days obtained from xenotrans‐planted tumors in nude mice. The numbers of chromosomes 7 and 17 analyzed with the karyotypic preparations coincided well with those determined by FISH, even in the paraffin‐embedded specimens. The number of tumor cells showing no signals, however, increased in the specimens after 5 days formalin fixation. In 10 surgically removed gastric carcinomas, the predominant signal number for chromosomes 7 and 17 in the cells of paraffinembedded tissues was two (disomy), except in one papillary carcinoma, which was trisomic for chromosome 7. Large subpopulations (more than 20%) showing trisomy were found in four cases for chromosome 7 and in five cases for chromosome 17. A higher frequency of trisomy was found in well differentiated than in poorly differentiated carcinomas. These findings suggest that the FISH technique is a useful tool for detecting chromosomal aberrations in gastric adenocarcinoma cells, even in paraffinembedded specimens, as long as the tissues are fixed with formalin for an appropriate time.

Interphase cytogenetics in paraffin sections of lung tumors by non-isotopic in situ hybridization. Mapping genotype/phenotype heterogeneity.