Comparative physiological and transcriptomic analyses illuminate common mechanisms by which silicon alleviates cadmium and arsenic toxicity in rice seedlings
Comparative physiological and transcriptomic analyses illuminate common mechanisms by which silicon alleviates cadmium and arsenic toxicity in rice seedlings
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比较生理学和转录组分析阐明了硅减轻稻苗镉和砷毒性的常见机制
DOI：
10.1016/j.jes.2021.02.030
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发表时间：
2021-11-01
影响因子：
6.9
通讯作者：
Yi, Jicai
中科院分区：
文献类型：
--
作者：
Chen, Huiqiong;Liang, Xiaoyu;Yi, Jicai
非必需重金属镉（Cd）和砷（As）常共存于污染水稻土中，对水稻具有毒害作用。硅（Si）处理可以降低水稻植株中Cd和As的毒性。为了更好地了解硅缓解镉和砷胁迫的共同机制，水稻幼苗水培暴露于镉或砷，然后用硅处理。硅的添加显著改善了镉和砷对水稻幼苗生长的抑制效应。硅处理降低了Cd和As从根向地上部的转运，并显著降低了Cd和As诱导的水稻幼苗活性氧的产生。本研究通过转录组学分析阐明了Si介导的水稻对Cd或As胁迫响应的分子机制。比较了施硅和不施硅条件下镉和砷胁迫下水稻根系差异表达基因的表达模式。镉和砷胁迫水稻根的转录组同样和深刻重塑硅的应用，这表明硅可能发挥了重要的，积极的作用，在植物防御重金属胁迫，通过调节全基因组的表达。我们还确定了两个新的基因，0 s 01 g 0524500和0 s 06 g 0514800，分别编码成髓细胞瘤（MYB）转录因子和硫素，这可能是候选目标硅缓解镉和砷胁迫水稻，以及镉和/或砷抗性植物的产生。本研究为进一步阐明硅介导的减轻水稻镉和砷毒害的共同分子机制提供了宝贵的资源。(c)2021中国科学院生态环境科学研究中心由Elsevier B. V.出版。这是一篇开放获取的文章，使用CC BY-NC-ND许可证（http://creativecommons.org/licenses/by-nc-nd/4.0/）
The inessential heavy metalloids cadmium (Cd) and arsenic (As), which often co-occur in polluted paddy soils, are toxic to rice. Silicon (Si) treatment is known to reduce Cd and As toxicity in rice plants. To better understand the shared mechanisms by which Si alleviates Cd and As stress, rice seedlings were hydroponically exposed to Cd or As, then treated with Si. The addition of Si significantly ameliorated the inhibitory effects of Cd and As on rice seedling growth. Si supplementation decreased Cd and As translocation from roots to shoots, and significantly reduced Cd- and As-induced reactive oxygen species generation in rice seedlings. Transcriptomics analyses were conducted to elucidate molecular mechanisms underlying the Si-mediated response to Cd or As stress in rice. The expression patterns of the differentially expressed genes in Cd- or As-stressed rice roots with and without Si application were compared. The transcriptomes of the Cd- and As-stressed rice roots were similarly and profoundly reshaped by Si application, suggesting that Si may play a fundamental, active role in plant defense against heavy metalloid stresses by modulating whole genome expression. We also identified two novel genes, 0s01g0524500 and 0s06g0514800 , encoding a myeloblastosis (MYB) transcription factor and a thionin, respectively, which may be candidate targets for Si to alleviate Cd and As stress in rice, as well as for the generation of Cd- and/or As-resistant plants. This study provides valuable resources for further clarification of the shared molecular mechanisms underlying the Si-mediated alleviation of Cd and As toxicity in rice. (c) 2021 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )