Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy

Optical imaging of non-fluorescent nanodiamonds in live cells using transient absorption microscopy
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DOI:
10.1039/c3nr00308f
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发表时间:
2013-01-01
期刊:
影响因子:
6.7
通讯作者:
Huang, Yanyi
Huang, Yanyi
中科院分区:
材料科学2区
文献类型:
--
作者:
Chen, Tao;Lu, Feng;Huang, Yanyi

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我们使用瞬态吸收显微镜直接观察活细胞中的非荧光纳米金刚石。这种无标记技术提供了一种新的模式来研究具有内在三维成像能力的细胞内纳米金刚石的动态行为。我们应用这种方法来捕获各种条件下纳米金刚石的细胞摄取,证实了内吞机制。
We directly observe non-fluorescent nanodiamonds in living cells using transient absorption microscopy. This label-free technology provides a novel modality to study the dynamic behavior of nanodiamonds inside the cells with intrinsic three-dimensional imaging capability. We apply this method to capture the cellular uptake of nanodiamonds under various conditions, confirming the endocytosis mechanism.