Aminobacterium colombiense gen. nov. sp. nov., an amino acid-degrading anaerobe isolated from anaerobic sludge
Aminobacterium colombiense gen. nov. sp. nov., an amino acid-degrading anaerobe isolated from anaerobic sludge
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DOI：
10.1006/anae.1998.0170
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发表时间：
1998-10-01
期刊：
影响因子：
2.3
通讯作者：
Patel, BKC
中科院分区：
文献类型：
--
作者：
Baena, S;Fardeau, ML;Patel, BKC
从某乳业废水处理厂厌氧泻湖中分离到一株新的革兰氏阴性、不产孢、嗜中温、氨基酸发酵菌，命名为菌株ALA-1(T) （T =型菌株）。应变是弯曲的（3-4 μ m x 0.2-0.3 μ m），可以单独或成对出现。最佳生长条件为37℃和pH值7.3。DNA的G+C含量为46mol %。该菌株需要酵母提取物生长，在酪胺酸、蛋白胨、半胱氨酸和α -酮戊二酸盐上生长不良，但在丝氨酸、苏氨酸、甘氨酸和丙酮酸盐上生长良好。当与氢营养产甲烷菌甲酸甲烷杆菌共培养时，菌株ALA-1(T)氧化丙氨酸、谷氨酸、亮氨酸、异亮氨酸、缬氨酸、天冬氨酸和蛋氨酸。系统发育分析表明，该菌株与低G + C的革兰氏阳性菌Dethiosulfovibrio peptidovorans、Dictyoglomus thermoophilum和Anaerobaculum thermoterrenum形成了一个独特而独立的谱系。系统发育结果与表型和基因组数据一致，显示它是一个新的菌株。基于这些发现，我们将菌株ALA-1T命名为哥伦比亚氨基细菌（DSM 12261） gen. nov. sp. nov. (C) 1998学术出版社。
A new Gram-negative, non-sporulating, mesophilic, amino acid-fermenting bacterium, designated strain ALA-1(T) (T = type strain), was isolated from an anaerobic lagoon of a dairy wastewater treatment plant. The strain is curved (3-4 mu m x 0.2-0.3 mu m) and occurs singly or in pairs. Optimum growth occurs at 37 degrees C and pH 7.3. The G+C content of the DNA is 46mol %. The strain requires yeast extract for growth, grows poorly on casamino acids, peptones, cystein, and alpha-ketoglutarate, but readily grows on serine, threonine, glycine and pyruvate. When cocultured with the hydrogenotrophic methanogen Methanobacterium formicicum, strain ALA-1(T) oxidized alanine, glutamate, leucine, isoleucine, valine, aspartate, and methionine. Phylogenetic analysis revealed that it forms a distinct and independent line of descent in the vicinity of Dethiosulfovibrio peptidovorans, Dictyoglomus thermophilum, and Anaerobaculum thermoterrenum which are members of the low G + C containing Gram-positive bacteria. The phylogenetic results concur with the phenotypic and genomic data which reveal that it is a novel strain. Based on these findings, we designate strain ALA-1T as Aminobacterium colombiense (DSM 12261) gen. nov., sp. nov. (C) 1998 Academic Press.