CHARACTERIZATION OF AN ASPERGILLUS-NIDULANS L-ARABITOL DEHYDROGENASE MUTANT
CHARACTERIZATION OF AN ASPERGILLUS-NIDULANS L-ARABITOL DEHYDROGENASE MUTANT
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DOI：
10.1016/0378-1097(94)90278-x
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发表时间：
1994-10-15
影响因子：
2.1
通讯作者：
VISSER, J
中科院分区：
文献类型：
--
作者：
DEVRIES, RP;FLIPPHI, MJA;VISSER, J
l -阿拉伯糖的降解途径包括在最终磷酸化之前的一系列交替还原和氧化反应，研究了在野生型和l -阿拉伯糖非利用突变体中进行的研究。突变体不能利用l -阿拉伯糖是由于缺乏l -阿拉伯糖醇脱氢酶活性造成的。在不同的碳源上研究了突变对多元醇积累模式的影响。在这个突变体中，l -阿拉伯糖的存在导致了阿拉伯糖醇在细胞内的积累。此外，在这些条件下，突变体分泌阿拉伯糖醇，与野生型相比，参与l -阿拉伯糖分解代谢的酶以及参与降解植物细胞壁多糖l -阿拉伯糖的细胞外糖基水解酶的表达增强。
The degradation pathway for L-arabinose, which consists of a sequence of alternating reduction and oxidation reactions prior to ultimate phosphorylation, was studied in Aspergillus nidulans wild-type as well as in an L-arabinose non-utilizing mutant. The inability of the mutant to use L-arabinose was caused by the absence of L-arabitol dehydrogenase activity. The effect of the mutation on polyol accumulation patterns was studied upon growth on various carbon sources. The presence of L-arabinose resulted in intracellular accumulation of arabitol in this mutant. Moreover, the mutant secreted arabitol under these conditions and, in contrast to the wild-type, featured enhanced expression of enzymes involved in L-arabinose catabolism as well as of extracellular glycosyl hydrolases involved in degradation of the plant cell wall polysaccharide L-arabinan.