Prevalence of major enteric pathogens in Australian dairy calves with diarrhoea
Prevalence of major enteric pathogens in Australian dairy calves with diarrhoea
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DOI：
10.1111/j.1751-0813.2011.00692.x
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发表时间：
2011-05-01
影响因子：
1.1
通讯作者：
House, J. K.
中科院分区：
文献类型：
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作者：
Izzo, M. M.;Kirkland, P. D.;House, J. K.
目的确定主要肠道病原体的流行情况，在奶牛和乳牛小牛腹泻在Australia.Design横断面study.Methods粪便样本从84个澳大利亚奶牛和乳牛物业（597个样本）进行了筛选轮状病毒和冠状病毒，使用实时逆转录聚合酶链反应，沙门氏菌属。使用选择性富集粪便培养物，以及使用商业酶联免疫吸附测定法用于产肠毒素大肠杆菌（K99）和隐孢子虫。结果97.6%的暴发疫情和95.0%的样品分离出肠道致病菌。轮状病毒是最常见的病原体（477/597，79.9%），其次是C。细小沙门氏菌（349/597，58.5%），沙门氏菌属（349/597，58.5%）。(142/597，23.8%）、冠状病毒（129/597，21.6%）和E.大肠杆菌K99（104/597，17.4%）。在96.4%的农场和71.0%的样品中鉴定出多种病原体。来自奶牛场的样品比来自乳制品场的样品更容易有多种病原体（P < 0.05），而轮状病毒和沙门氏菌属。结论奶牛场和奶牛场的犊牛腹泻病暴发大多涉及多种病原体。轮状病毒和C.在整个生产系统中，最常见的病原体是细小病毒。沙门氏菌和轮状病毒在奶牛肉生产中更常见。
Objective Determine the prevalence of the major enteric pathogens in dairy and dairy beef calves with diarrhoea in Australia.Design Cross-sectional study.Methods Faecal samples from 84 Australian dairy and dairy beef properties (597 samples) were screened for rotavirus and coronavirus using real-time reverse transcription polymerase chain reaction, for Salmonella spp. using selective enrichment faecal culture, and for enterotoxigenic Escherichia coli (K99) and Cryptosporidium parvum using a commercial enzyme-linked immunosorbent assay. A logistic regression with random effects model was used to compare prevalence of pathogens in dairy and dairy beef operations.Results Enteric pathogens were isolated from 97.6% of outbreaks and 95.0% of samples. Rotavirus was the most common pathogen identified (477/597, 79.9%) followed by C. parvum (349/597, 58.5%), Salmonella spp. (142/597, 23.8%), coronavirus (129/597, 21.6%) and E. coli K99 (104/597, 17.4%). Multiple pathogens were identified on 96.4% of farms and from 71.0% of samples. Samples from dairy beef properties were more likely to have multiple pathogens than dairy properties (P < 0.05), whereas rotavirus and Salmonella spp. were more likely to be identified in samples collected from dairy beef than dairy properties (P < 0.05).Conclusion Most outbreaks of calf diarrhoea in dairy and dairy beef operations involve multiple pathogens. Rotavirus and C. parvum were the most frequently identified pathogens across production systems. Salmonella spp. and rotavirus were more frequently identified in dairy beef operations.