Transmission of Helicobacter pylori between a human and two dogs: A case report
Transmission of Helicobacter pylori between a human and two dogs: A case report
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DOI：
10.1111/hel.12798
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发表时间：
2021-04-05
期刊：
影响因子：
4.4
通讯作者：
Tsujimoto, Hajime
中科院分区：
文献类型：
--
作者：
Kubota-Aizawa, Sanae;Matsubara, Yasuo;Tsujimoto, Hajime
虽然非幽门螺杆菌作为人畜共患病的来源，经常在狗和猫的胃中被检测到，已经引起了相当大的关注，但宠物在幽门螺杆菌流行病学中的作用尚不清楚。在我们之前的研究中，在一只狗（狗1）的胃中检测到幽门螺杆菌感染。在这里，我们调查了同一家庭中狗1（狗2）的雌性后代及其主人的幽门螺杆菌感染状况。材料与方法从狗主人处取活检标本，进行幽门螺杆菌检测。从狗1的胃活检样本、狗2的胃液沉淀物和主人的胃中提取DNA，并进行幽门螺杆菌属和种特异性pcr。然后，对ureAB基因的部分区域进行了序列分析。结果犬和主人的标本属特异性PCR反应均为阳性，而felis Helicobacter -、bizzozeronii-和heilmannii Helicobacter sensu严格特异性PCR反应均为阴性。所有三个样品在幽门螺杆菌特异性PCR中也呈阳性反应。所有受试者的部分ureAB基因序列相同。结论这两只狗及其主人感染了同一株幽门螺旋杆菌。该报告首次证明幽门螺旋杆菌可以在人与狗之间传播。从预防流行病学角度探讨人犬间幽门螺杆菌传播的危险因素还有待进一步研究。
Background Whereas non-Helicobacter pylori helicobacters, which are frequently detected in the stomachs of dogs and cats as a source of zoonoses, have attracted considerable attention, the role of pets in H. pylori epidemiology is unclear. In our previous study, an H. pylori infection was detected in the stomach of a dog (Dog 1). Here, we investigated the H. pylori infection status in the female offspring of Dog 1 (Dog 2) and its owner within the same household.Materials and Methods Biopsy specimens were obtained from the dog's owner and tested for H. pylori. DNA from gastric biopsy samples of Dog 1, gastric fluid sediment of Dog 2, and bacteria from the stomach of the owner was obtained, and Helicobacter genus- and species-specific PCRs were performed. Then, sequence analyses of the partial region of the ureAB gene were conducted.Results Samples from both dogs and the owner reacted positively in the genus-specific PCR and negative in the Helicobacter felis-, Helicobacter bizzozeronii-, and Helicobacter heilmannii sensu stricto-specific PCRs. All three samples also reacted positively in the H. pylori-specific PCR. Sequences of the partial ureAB gene from all subjects were identical.Conclusions The results suggested that the two dogs and their owner were infected with an identical H. pylori strain. This report is the first to demonstrate that H. pylori can be transmitted between humans and dogs. Further studies are required to investigate the risk factors for the transmission of H. pylori between humans and dogs from the perspective of preventive epidemiology.