Identification and characterization of Mini1, a gene regulating rice shoot development
Identification and characterization of Mini1, a gene regulating rice shoot development
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水稻芽发育调控基因 Mini1 的鉴定和表征
DOI：
10.1111/jipb.12230
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发表时间：
2015-02-01
影响因子：
11.4
通讯作者：
Qian, Qian
中科院分区：
文献类型：
--
作者：
Fang, Yunxia;Hu, Jiang;Qian, Qian
高等植物的地上部分是由茎顶端分生组织（SAM）产生的。本研究从水稻（Oryza sativa L.）突变体表现出芽发育的提前终止，并被命名为mini rice 1（mini 1）。该突变体是首次从粳稻品种中花11号（ZH 11）中分离到的经甲基磺酸乙酯（EMS）处理的突变体。采用群体分离分析和图位克隆的方法，将Mini 1基因定位在第9染色体上48.6kb的区域内。序列分析表明，该区域中发现了一个由G到A的单碱基替换，导致氨基酸由Gly变为Asp。候选基因Os 09 g 0363900被预测编码一个推测的萼缘粘附蛋白ACE（推测的HOTHEAD前体），遗传互补实验证实了Mini 1的身份。Os 09 g 0363900含有葡萄糖-甲醇-胆碱（glucose-methanol-choline，GMC）氧化还原酶和NAD（P）结合Rossmann样结构域，与拟南芥HOTHEAD（Arabidopsis HOTHEAD，HTH）具有高度的相似性。表达分析表明，Mini 1在幼芽中表达量较高，在根中表达量较低，与生长素合成和信号转导有关的基因在突变体中的表达量降低。因此，Mini 1在维持水稻SAM活性和促进地上部发育方面起着重要作用。
The aerial parts of higher plants are generated from the shoot apical meristem (SAM). In this study, we isolated a small rice (Oryza sativa L.) mutant that showed premature termination of shoot development and was named mini rice 1 (mini1). The mutant was first isolated from a japonica cultivar Zhonghua11 (ZH11) subjected to ethyl methanesulfonate (EMS) treatment. With bulked segregant analysis (BSA) and map-based cloning method, Mini1 gene was finally fine-mapped to an interval of 48.6kb on chromosome 9. Sequence analyses revealed a single base substitution from G to A was found in the region, which resulted in an amino acid change from Gly to Asp. The candidate gene Os09g0363900 was predicted to encode a putative adhesion of calyx edges protein ACE (putative HOTHEAD precursor) and genetic complementation experiment confirmed the identity of Mini1. Os09g0363900 contains glucose-methanol-choline (GMC) oxidoreductase and NAD(P)-binding Rossmann-like domain, and exhibits high similarity to Arabidopsis HOTHEAD (HTH). Expression analysis indicated Mini1 was highly expressed in young shoots but lowly in roots and the expression level of most genes involved in auxin biosynthesis and signal transduction were reduced in mutant. We conclude that Mini1 plays an important role in maintaining SAM activity and promoting shoot development in rice.