Spatial structure of mitochondria and ER denotes changes in cell physiology of cultured tobacco protoplasts
Spatial structure of mitochondria and ER denotes changes in cell physiology of cultured tobacco protoplasts
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线粒体和 ER 的空间结构表示培养的烟草原生质体细胞生理学的变化
DOI：
10.1046/j.1365-313x.1996.09010085.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
J. Verbelen
中科院分区：
文献类型：
--
作者：
D. Stickens;J. Verbelen
用DiOC 6（3）（3，3 ′-dihexyloxacarbocyanine iodide）染料和共聚焦激光扫描显微镜（CLSM）研究了叶肉原生质体和再生细胞中线粒体和内质网的结构。使用基于细胞的模型系统的伸长和分裂的细胞的生理学的关系进行了研究。线粒体的结构及其数量取决于细胞的状态。在新鲜分离的原生质体中，小的球形线粒体聚集在细胞核和叶绿体周围。在培养的前4天，它们转化为均匀分布在整个细胞质中的长蠕虫状细胞器。在仅含有生长素的培养基中，细胞然后进入扩增期。它们的线粒体保持相同的结构，但数量增加。在含有生长素和细胞分裂素的培养基中，细胞开始分裂。它们的线粒体通常变得非常多和非常小，并且分布在整个细胞质中。这两种类型的组织都是在持续扩张或分裂的几周内观察到的。ER总是以靠近细胞膜的网络形式存在。在新鲜分离的原生质体中，相当一部分ER聚集在叶绿体周围，网络的其余部分具有降低的复杂性并且部分被破坏。在随后的原生质体培养过程中，该网络生长成一个复杂的网状结构，其中包含许多板状结构。这在伸长细胞和分裂细胞中都是如此。最后，ER看起来与完整植物的表皮细胞中发现的结构相似。
The structure of mitochondria and of the endoplasmic reticulum (ER) in mesophyll protoplasts and regenerated cells was studied in vivo using the dye DiOC6(3) (3,3′-dihexyloxacarbocyanine iodide) and confocal laser scanning microscopy (CLSM). The relation to the cell’s physiology was investigated using a hormone-based model system for elongation and division. The structure of the mitochondria and of their population depends on the status of the cell. In freshly isolated protoplasts small spherical mitochondria are clustered around the nucleus and the chloroplasts. During the first 4 days of culture they are transformed into long vermiform organelles which distribute evenly throughout the cytoplasm. In a medium containing only auxins, cells then enter a period of expansion. Their mitochondria retain the same structure but increase in quantity. In a medium with auxins and cytokinins cells start dividing. Their mitochondria typically become numerous and very small, and are distributed throughout the cytoplasm. Both types of organization were observed during weeks of ongoing expansion or division. The ER is always present as a network close to the cell membrane. In freshly isolated protoplasts a considerable part of the ER is clustered around the chloroplasts, the remaining part of the network has a reduced complexity and is partly broken. During subsequent protoplast culture the network grows into a complex web with fine meshes incorporating lots of plate-like structures. This is the case in elongating cells as well as in dividing cells. Finally, the ER looks similar to the structure found in epidermal cells of the intact plant.