TaNF-Y-PRC2 orchestrates temporal control of starch and protein synthesis in wheat
TaNF-Y-PRC2 orchestrates temporal control of starch and protein synthesis in wheat
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DOI：
10.1101/2023.12.26.573020
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发表时间：
2023-12
期刊：
影响因子：
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通讯作者：
Jinchao Chen;Long Zhao;Haoran Li;Changfeng Yang;Dongzhi Wang;Xuelei Lin;Yujing Lin;Hao Zhang-Hao-Z
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文献类型：
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作者：
Jinchao Chen;Long Zhao;Haoran Li;Changfeng Yang;Dongzhi Wang;Xuelei Lin;Yujing Lin;Hao Zhang-Hao-Z
谷物中的胚乳控制着淀粉和蛋白质的产生，在决定谷物产量和种子质量方面起着重要作用。在这项研究中，我们发现了一个特定的由tanf - y3 - d、TaNF-YB7-B和TaNF-YC6-B组成的TaNF-Y三聚体复合物，在小麦籽粒灌浆期在胚乳中表现出强劲的表达。敲除TaNF-YA3或TaNF-YC6都会导致淀粉减少，但麸质蛋白增加。详细分析表明，TaNF-Y通过降低TaAGPS1a、TaSuS2的抑制因子TaNAC019间接促进淀粉生物合成基因，从而调节淀粉生物合成。相反，TaNF-Y直接抑制麦胶蛋白和低分子量(LMW)-GS编码基因的表达，包括TaGli-γ-700和TaLMW-400。此外，TaNF-Y组分与Polycomb抑制复合体2 （PRC2）的组蛋白甲基转移酶亚基TaSWN相互作用，通过H3K27me3修饰抑制TaNAC019、TaGli-γ-700和TaLMW-400的表达。值得注意的是，PRC2核心亚基TaFIE的弱突变减少了淀粉，但升高了麦胶蛋白和LMW-GS水平。有趣的是，TaNF-Y组分的DNA变异与种子发育性状广泛相关。特别是，编码区域内的TaNF-YB7-B的变异与淀粉和蛋白质含量的差异有关。TaNF-YB7-B的不同单倍型影响其与TaSWN的相互作用，从而影响TaNAC019和TaGli-γ-700等靶标的抑制。我们的发现阐明了小麦胚乳发育中由TaNF-Y-PRC2调控表观遗传的复杂分子机制。操纵TaNF-Y复合物具有优化粮食产量和提高品质的潜力。
The endosperm in cereal grains is instrumental in determining grain yield and seed quality, as it controls the production of starch and protein. In this study, we identified a specific TaNF-Y trimeric complex, consisting of TaNF-YA3-D, TaNF-YB7-B, and TaNF-YC6-B, exhibiting robust expression within endosperm during grain filling stage in wheat. Knock-down of either TaNF-YA3 or TaNF-YC6 led to less starch but more gluten proteins. Detailed analyses have unveiled that the TaNF-Y indirectly boosts starch biosynthesis genes by reducing TaNAC019, a repressor of TaAGPS1a, TaSuS2, thereby regulating starch biosynthesis. Conversely, the TaNF-Y directly inhibits the expression of gliadin and low molecular weight (LMW)-GS coding genes, including TaGli-γ-700 and TaLMW-400. Furthermore, the TaNF-Y components interact with TaSWN, the histone methyltransferase subunit of Polycomb repressive complex 2 (PRC2), to repress the expression of TaNAC019, TaGli-γ-700, and TaLMW-400 through H3K27me3 modification. Notably, weak mutation of TaFIE, core subunit of PRC2, has reduced starch but elevated gliadin and LMW-GS levels. Intriguingly, DNA variations of TaNF-Y components are widely associated with seed developmental traits. In particular, variation within the coding region of TaNF-YB7-B is linked to differences in starch and protein content. Distinct haplotypes of TaNF-YB7-B affect its interaction with TaSWN, influencing the repression of targets like TaNAC019 and TaGli-γ-700. Our findings illuminate the intricate molecular mechanisms governing epigenetic regulation by the TaNF-Y-PRC2 for wheat endosperm development. Manipulating the TaNF-Y complex holds potential for optimizing grain yield and enhancing quality.