Effect of low‐intensity pulsed ultrasound on orofacial sensory disturbance following inferior alveolar nerve injury: Role of neurotrophin‐3 signaling
Effect of low‐intensity pulsed ultrasound on orofacial sensory disturbance following inferior alveolar nerve injury: Role of neurotrophin‐3 signaling
复制标题
低强度脉冲超声对下牙槽神经损伤后口面部感觉障碍的影响：神经营养素-3信号传导的作用
DOI：
10.1111/eos.12810
复制
发表时间：
2021
影响因子：
1.9
通讯作者：
Shinoda Masamichi
中科院分区：
文献类型：
--
作者：
Tsuchimochi Akane;Endo Chitose;Motoyoshi Mitsuru;Tamura Miki;Hitomi Suzuro;Hayashi Yoshinori;Shinoda Masamichi
低强度脉冲超声（LIPUS）经皮治疗下牙槽神经（IAN）横断部位可促进功能再生，但详细机制尚不清楚。我们研究了神经营养素-3（NT-3）（主要与原肌球蛋白受体激酶C（TrkC）结合）在大鼠LIPUS治疗后功能性横断IAN再生中的作用。每天对切断的IAN进行LIPUS治疗，并测量面部皮肤的机械敏感性，持续14 d。在IAN切断后第5天，用免疫组织化学方法检测切断的IAN和TrkC阳性三叉神经节神经元中NT-3的表达。此外，还分析了TrkC中和对LIPUS治疗加速面部机械感觉障碍恢复的影响。对IAN横断部位进行LIPUS治疗显著促进了面部皮肤感觉障碍的功能恢复。横断IAN中的雪旺细胞表达NT-3，LIPUS处理增加了NT-3的量。通过连续LIPUS治疗促进的机械感觉障碍的恢复受到IAN横断部位持续TrkC中和的抑制。这些结果表明，LIPUS治疗通过增强切断的IAN中的NT-3信号来加速IAN切断后口面机械感觉功能的恢复。
Percutaneous treatment of low‐intensity pulsed ultrasound (LIPUS) to the site of inferior alveolar nerve (IAN) transection promotes functional regeneration, but the detailed mechanism is unknown. We examined the involvement of neurotrophin‐3 (NT‐3), which primarily binds with tropomyosin receptor kinase C (TrkC), in functional transected IAN regeneration following LIPUS treatment in rats. Daily LIPUS treatment to the transected IAN was performed, and the mechanical sensitivity of the facial skin was measured for 14 d. On day 5 after IAN transection, the expression of NT‐3 in the transected IAN and TrkC‐positive trigeminal ganglion neurons were immunohistochemically examined. Further, the effect of TrkC neutralization on the acceleration of facial mechanosensory disturbance restoration due to LIPUS treatment was analyzed. LIPUS treatment to the site of IAN transection significantly facilitated functional recovery from sensory disturbance on facial skin. Schwann cells in the transected IAN expressed NT‐3, and LIPUS treatment increased the amount of NT‐3. The facilitated recovery from the mechanosensory disturbance by continuous LIPUS treatment was inhibited by the ongoing TrkC neutralization at the IAN transection site. These results suggest that LIPUS treatment accelerates the recovery of orofacial mechanosensory function following IAN transection through the enhancement of NT‐3 signaling in the transected IAN.