Increased interleukin-8 expression in inflamed human dental pulps
Increased interleukin-8 expression in inflamed human dental pulps
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DOI：
10.1016/s1079-2104(99)70118-6
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发表时间：
1999-08-01
影响因子：
--
通讯作者：
Zhang, XL
中科院分区：
文献类型：
--
作者：
Huang, GTJ;Potente, AP;Zhang, XL
目的：白细胞介素8是一种强有力的趋化因子和中性粒细胞激活剂，其水平升高与感染性和炎症性疾病有关。然而，对白细胞介素8在人牙髓中的表达知之甚少。这项研究的目的是确定不可逆炎症的人牙髓组织中白细胞介素8的水平是否升高。实验样本来自临床诊断为不可逆性牙髓炎(病变牙髓)的牙齿。对照组为新鲜拔除的无龋齿第三磨牙(正常牙髓)。用IL-8特异性抗体进行酶联免疫吸附试验和/或免疫组织化学分析。酶联免疫吸附试验显示，病变牙髓中的白介素8水平(平均为1.82+/-0.79 pg/m L/µg蛋白质)高于正常牙髓样本中可检测到的白介素8水平(平均为0.08+/-0.04 pg/m L/m g蛋白质；P
Objective, Elevated levels of interleukin-8, a potent chemoattractant and activator of neutrophils, are associated with infectious and inflammatory diseases. However, little is known about interleukin-8 expression in human dental pulp. The purpose of this study was to determine whether tissue levels of interleukin-8 are elevated in irreversibly inflamed human pulps.Study design. Experimental samples were from teeth clinically diagnosed with irreversible pulpitis (diseased pulps). Controls were from freshly extracted, caries-free third molars (normal pulps). Samples were subjected to enzyme-linked immunosorbent assay and/or immunohistochemical analysis with specific antibodies to interleukin-8.Results. The enzyme-linked immunosorbent assay studies showed elevated levels of interleukin-8 in diseased pulps (mean, 1.82 +/- 0.79 pg/mL/mu g protein), as compared to detectable interleukin-8 levels in samples from normal pulps (mean, 0.08 +/- 0.04 pg/mL/mu g protein; P