Developmental changes in cell proliferation in the auditory midbrain of the bullfrog, Rana catesbeiana.
Developmental changes in cell proliferation in the auditory midbrain of the bullfrog, Rana catesbeiana.
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牛蛙听觉中脑细胞增殖的发育变化。
DOI：
10.1002/neu.20301
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Brown,RebeccaA
中科院分区：
文献类型：
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作者：
Simmons,AndreaMegela;Chapman,JudithA;Brown,RebeccaA
通过观察5-溴-2 ′-脱氧尿苷（BrdU）在循环细胞中的掺入，我们研究了牛蛙（Rana catesbeiana）在幼虫和早期变态后发育过程中听性中脑（枕叶）细胞增殖的模式。在所有发育阶段，BrdU标记的细胞集中在视脑室周围。BrdU标记的细胞也以阶段特异性的方式出现在隆突本身内。有丝分裂指数（定量为脑室区外BrdU阳性细胞/可用于标记的总细胞的百分比）随幼虫发育而变化。有丝分裂指数在幼体、早期幼虫和晚期幼虫阶段较低，在聋期、变态顶极期和幼蛙期显著升高。细胞增殖是较高的变态高潮比在其他阶段，这表明增加细胞增殖准备从水生过渡到两栖的存在。发育过程中有丝分裂指数的变化与可用于标记的细胞总数的变化不平行。另外通过斑点印迹法定量测定BrdU掺入，表明BrdU可用于标记，直至注射后72 h。在圆枕的细胞增殖的变化模式不同，在听觉延髓（背髓核和上级橄榄核），这表明，在这些不同的听觉核细胞增殖介导的不同的潜在机制。© 2006 Wiley Periodicals，Inc. J Neurobiol 66：1212-1224，2006
We examined patterns of cell proliferation in the auditory midbrain (torus semicircularis) of the bullfrog,Rana catesbeiana, over larval and early postmetamorphic development, by visualizing incorporation of 5‐bromo‐2′‐deoxyuridine (BrdU) in cycling cells. At all developmental stages, BrdU‐labeled cells were concentrated around the optic ventricle. BrdU‐labeled cells also appeared within the torus semicircularis itself, in a stage‐specific manner. The mitotic index, quantified as the percent of BrdU‐positive cells outside the ventricular zone per total cells available for label, varied over larval development. Mitotic index was low in hatchling, early larval, and late larval stages, and increased significantly in deaf period, metamorphic climax, and froglet stages. Cell proliferation was higher in metamorphic climax than at other stages, suggesting increased cell proliferation in preparation for the transition from an aquatic to an amphibious existence. The change in mitotic index over development did not parallel the change in the total numbers of cells available for label. BrdU incorporation was additionally quantified by dot‐blot assay, showing that BrdU is available for label up to 72 h postinjection. The pattern of change in cell proliferation in the torus semicircularis differs from that in the auditory medulla (dorsal medullary nucleus and superior olivary nucleus), suggesting that cell proliferation in these distinct auditory nuclei is mediated by different underlying mechanisms. © 2006 Wiley Periodicals, Inc. J Neurobiol 66: 1212–1224, 2006