Two-photon excitation fluorescence lifetime imaging microscopy: A promising diagnostic tool for digestive tract tumors
Two-photon excitation fluorescence lifetime imaging microscopy: A promising diagnostic tool for digestive tract tumors
复制标题
双光子激发荧光寿命成像显微镜：一种有前途的消化道肿瘤诊断工具
DOI：
10.1142/s179354581930009x
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发表时间：
2019-09-01
影响因子：
2.5
通讯作者：
Zheng, Wei
中科院分区：
文献类型：
--
作者：
Li, Hui;Yu, Jia;Zheng, Wei
消化道肿瘤分别占癌症发病率和死亡率的15%和19.3%。早期发现消化道肿瘤对减轻全球癌症负担至关重要。双光子激发荧光寿命成像显微镜(TP-Flim)是一种非侵入性、无标记、三维、高分辨率的生物组织成像技术，不仅具有组织学特性，而且具有生物化学定性和定量的能力。得益于这些优势，该技术在消化道肿瘤的临床诊断中具有广阔的应用前景。近年来，在这方面做了很多努力，取得了一些显著的进展。本文就TP-FILM在消化道肿瘤检测中的研究进展作一综述。其中，我们在胃癌和食道癌方面的最新成果被精心描绘。最后，我们展望和讨论了TP-FLIM在未来临床应用中的潜在优势和挑战。
Digestive tract tumors account for 15% and 19.3% of the cancer incidence and deaths, respectively. Early detection of digestive tract tumors is crucial to the reduction of global cancer burden. Two-photon excitation fluorescence lifetime imaging microscopy (TP-FLIM) allows non-invasive, label-free, three-dimensional, high-resolution imaging of living tissues with not only histological but also biochemical characterization ability in both qualitative and quantitative way. Benefiting from these advantages, this technology is promising for clinical diagnosis of digestive tract tumors. In recent years, many efforts have been made in this field and some remarkable progress has been achieved. In this paper, we overview the recent progress of TP-FLIM-based researches on digestive tract tumor detection. Among them, our latest results on the gastric cancer and esophageal cancer are elaborately depicted. Finally, we outlook and discuss the potential advantages and challenges of TP-FLIM in future clinical applications.