Bacterial composition of microbial mats in hot springs in Northern Patagonia: variations with seasons and temperature
Bacterial composition of microbial mats in hot springs in Northern Patagonia: variations with seasons and temperature
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DOI：
10.1007/s00792-012-0499-z
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发表时间：
2013-01-01
期刊：
影响因子：
2.9
通讯作者：
Diez, Beatriz
中科院分区：
文献类型：
--
作者：
Mackenzie, Roy;Pedros-Alio, Carlos;Diez, Beatriz
在巴塔哥尼亚的三个温泉（39-68 A度C）的微生物垫的细菌多样性的季节性变化进行了分析，使用文化独立的方法。三个主要的细菌群体中检测到的所有弹簧：门蓝藻和拟杆菌，和为了Thermales。变形菌、酸菌和绿色非硫菌也有少量检出，且仅在部分样品中检出。Porcelana温泉和间歇泉的优势蓝藻为嗜热丝状杂囊藻Mastigocladus，Cahuelm的优势蓝藻为Calothrix，其次为丝状非杂囊藻Leptolyngbya和Oscillatoria。拟杆菌在很宽的温度范围内被检测到，它们的相对丰度随着温度的降低而增加，几乎所有的样品。两个不同的温度最适的Meiothermus种群被发现。总体而言，指纹图谱分析与通用细菌引物显示，在每个温泉，尽管温度的差异很高的相似性。另一方面，Cahuelm温泉在样本中显示出较低的相似性。Porcelana温泉和Porcelana间歇泉彼此相当相似，可能是由于地理位置接近的共同地质基质。这是更明显的与特定的蓝藻引物。不同的地质基质和海水的影响，在Cahuelm可能造成的微生物群落结构与其他两个温泉的差异。
Seasonal shifts in bacterial diversity of microbial mats were analyzed in three hot springs (39-68 A degrees C) of Patagonia, using culture-independent methods. Three major bacterial groups were detected in all springs: Phyla Cyanobacteria and Bacteroidetes, and Order Thermales. Proteobacteria, Acidobacteria and Green Non-Sulfur Bacteria were also detected in small amounts and only in some samples. Thermophilic filamentous heterocyst-containing Mastigocladus were dominant Cyanobacteria in Porcelana Hot Spring and Geyser, and Calothrix in Cahuelm, followed by the filamentous non-heterocyst Leptolyngbya and Oscillatoria. Bacteroidetes were detected in a wide temperature range and their relative abundance increased with decreasing temperature in almost all samples. Two Meiothermus populations with different temperature optima were found. Overall, fingerprinting analysis with universal bacterial primers showed high similarities within each hot spring despite differences in temperature. On the other hand, Cahuelm Hot Spring showed a lower resemblance among samples. Porcelana Hot Spring and Porcelana Geyser were rather similar to each other, possibly due to a common geological substrate given their geographic proximity. This was even more evident with specific cyanobacterial primers. The different geological substrate and the seawater influence in Cahuelm might have caused the differences in the microbial community structure with the other two hot springs.