Dwarf 88, a novel putative esterase gene affecting architecture of rice plant
Dwarf 88, a novel putative esterase gene affecting architecture of rice plant
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DOI：
10.1007/s11103-009-9522-x
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发表时间：
2009-10-01
影响因子：
5.1
通讯作者：
Han, Bin
中科院分区：
文献类型：
--
作者：
Gao, Zhenyu;Qian, Qian;Han, Bin
水稻构型是影响产量的重要农艺性状。本研究鉴定了一个水稻分蘖矮化突变体d88。用EMS处理粳稻品种兰生。与野生型相比，突变体具有过度短的分蘖和较小的穗和种子。d88突变体茎髓腔周围薄壁细胞数量减少，体积缩小，薄壁细胞伸长延迟，导致分蘖纤细，植株矮化。通过图位克隆分离D88基因，并鉴定其编码推定的酯酶。该基因在水稻大部分器官中表达，在维管组织中表达量特别高。该突变体在基因的第一个外显子中进行了核苷酸取代，导致精氨酸取代甘氨酸，这可能破坏了蛋白质的功能保守的N-豆蔻酰化结构域。通过互补试验和反义分析证实了该基因的功能。因此，D88代表了一类新的基因，它们调节细胞生长和器官发育，从而调节植物结构。分蘖形成相关基因和D88之间的潜在关系进行了讨论和未来的识别D88的基板可能会导致表征新的途径调节植物发育。
Rice architecture is an important agronomic trait that affects grain yield. We characterized a tillering dwarf mutant d88 derived from Oryza sativa ssp. japonica cultivar Lansheng treated with EMS. The mutant had excessive shorter tillers and smaller panicles and seeds compared to the wild-type. A reduction in number and size of parenchyma cells around stem marrow cavity as well as a delay in the elongation of parenchyma cells caused slender tillers and dwarfism in the d88 mutant. The D88 gene was isolated via map-based cloning and identified to encode a putative esterase. The gene was expressed in most rice organs, with especially high levels in the vascular tissues. The mutant carried a nucleotide substitution in the first exon of the gene that led to the substitution of arginine for glycine, which presumably disrupted the functionally conserved N-myristoylation domain of the protein. The function of the gene was confirmed by complementation test and antisense analysis. D88, thus, represents a new category of genes that regulates cell growth and organ development and consequently plant architecture. The potential relationship between the tiller formation associated genes and D88 is discussed and future identification of the substrate for D88 may lead to the characterization of new pathways regulating plant development.