IDENTIFICATION OF THE HUMAN U7 SNRNP AS ONE OF SEVERAL FACTORS INVOLVED IN THE 3' END MATURATION OF HISTONE PREMESSENGER RNAS
IDENTIFICATION OF THE HUMAN U7 SNRNP AS ONE OF SEVERAL FACTORS INVOLVED IN THE 3' END MATURATION OF HISTONE PREMESSENGER RNAS
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DOI：
10.1126/science.2825355
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发表时间：
1987-12-18
期刊：
影响因子：
56.9
通讯作者：
STEITZ, JA
中科院分区：
文献类型：
--
作者：
MOWRY, KL;STEITZ, JA
在真核细胞中，基因转录物转化为信使RNA涉及多种因素，包括介导剪接反应的高度丰富的小核核糖核蛋白（snRNP）复合物。可分离因子也是组蛋白前mRNA的3“末端加工所必需的。3“切割位点侧翼的两个保守信号被活性HeLa细胞提取物中存在的离散组分识别：上游茎环与核酸酶不敏感因子相关，而与下游元件的结合由具有snRNP性质的组分介导。低丰度人U7 snRNP的RNA部分的序列表明哺乳动物前mRNA的相对简并下游元件如何通过RNA碱基配对被识别。
In eukaryotic cells, the conversion of gene transcripts into messenger RNA''s involves multiple factors, including the highly abundant small nuclear ribonucleoprotein (snRNP) complexes that mediate the splicing reaction. Separable factors are also required for the 3'' end processing of histone pre-mRNA''s. The two conserved signals flanking the 3'' cleavage site are recognized by discrete components present in active HeLa cell extracts: the upstream stem loop associates with a nuclease-insensitive factor, while binding to the downstream element is mediated by a component having the properties of a snRNP. The sequence of the RNA moiety of the low abundance human U7 snRNP suggests how the relatively degenerate downstream element of mammalian pre-mRNA''s could be recognized by RNA base-pairing.